PMID- 11273025
OWN - NLM
STAT- MEDLINE
DCOM- 20010614
LR  - 20191210
IS  - 0022-3573 (Print)
IS  - 0022-3573 (Linking)
VI  - 53
IP  - 2
DP  - 2001 Feb
TI  - A sensitive and reliable method for the detection of lipid peroxidation in 
      biological tissues.
PG  - 263-6
AB  - A simple, accurate and cost effective method has been designed for the 
      determination of lipid peroxidation in biological tissue samples. The method was 
      a modification and improvement on existing methods available for lipid 
      peroxidation determination. Solid-phase extraction was used to separate the 
      thiobarbituric acid-malondialdehyde complex from thiobarbituric acid-reactive 
      substances and HPLC was performed using a C18 (Waters Spherisorb, 5 microm, 250 x 
      4.6 mm i.d.) column to achieve isolation of the complex. The procedure was 
      validated with respect to linearity of calibration (0.998), precision, 
      sensitivity and limits of quantitation (1 nmol mL(-1)) and detection (0.5 nmol 
      mL(-1)). Resorcinol was used as an external standard. The method was tested by 
      inducing free radical generation with a known free radical generator, quinolinic 
      acid, in rat brain homogenate. The results showed that the method presented 
      allowed detection of lipid peroxidation products at concentrations in the 
      nanomolar (nM) range compared with the micromolar (microM) range detected by 
      other methods, thus rendering it suitable for use with biological samples. In 
      addition, the modified method allowed for detection of the purified lipid 
      peroxidation products, thus eliminating the possibility of simultaneous detection 
      of impurities that absorb at the same wavelength.
FAU - Anoopkumar-Dukie, S
AU  - Anoopkumar-Dukie S
AD  - Faculty of Pharmacy, Rhodes University, Grahamstown, South Africa.
FAU - Walker, R B
AU  - Walker RB
FAU - Daya, S
AU  - Daya S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
PL  - England
TA  - J Pharm Pharmacol
JT  - The Journal of pharmacy and pharmacology
JID - 0376363
RN  - 0 (Indicators and Reagents)
RN  - 0 (Neurotoxins)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - F6F0HK1URN (Quinolinic Acid)
SB  - IM
MH  - Animals
MH  - Brain Chemistry/*drug effects
MH  - Indicators and Reagents
MH  - Lipid Peroxidation/*drug effects
MH  - Male
MH  - Malondialdehyde/analysis
MH  - Neurotoxins/toxicity
MH  - Quinolinic Acid/toxicity
MH  - Rats
MH  - Rats, Wistar
MH  - Thiobarbituric Acid Reactive Substances/analysis
EDAT- 2001/03/29 10:00
MHDA- 2001/06/15 10:01
CRDT- 2001/03/29 10:00
PHST- 2001/03/29 10:00 [pubmed]
PHST- 2001/06/15 10:01 [medline]
PHST- 2001/03/29 10:00 [entrez]
AID - 10.1211/0022357011775299 [doi]
PST - ppublish
SO  - J Pharm Pharmacol. 2001 Feb;53(2):263-6. doi: 10.1211/0022357011775299.