PMID- 11274167
OWN - NLM
STAT- MEDLINE
DCOM- 20010712
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 276
IP  - 23
DP  - 2001 Jun 8
TI  - Targeted and extended acetylation of histones H4 and H3 at active and inactive 
      genes in chicken embryo erythrocytes.
PG  - 20197-205
AB  - Affinity-purified polyclonal antibodies recognizing the most highly acetylated 
      forms of histones H3 and H4 were used in immunoprecipitation assays with 
      chromatin fragments derived from 15-day chicken embryo erythrocytes by 
      micrococcal nuclease digestion. The distribution of hyperacetylated H4 and H3 was 
      mapped at the housekeeping gene, glyceraldehyde 3-phosphate dehydrogenase 
      (GAPDH), and the tissue-specific gene, carbonic anhydrase (CA). H3 and H4 
      acetylation was found targeted to the CpG island region at the 5' end of both 
      these genes, falling off in the downstream direction. In contrast, at the 
      beta(A)-globin gene, both H3 and H4 are highly acetylated throughout the gene and 
      at the downstream enhancer, with a maximum at the promoter. Low level acetylation 
      was observed at the 5' end of the inactive ovalbumin gene. Run-on assays to 
      measure ongoing transcription showed that the GAPDH and CA genes are transcribed 
      at a much lower rate than the adult beta(A)-globin gene. The extensive high level 
      acetylation at the beta(A)-globin gene correlates most simply with its high rate 
      of transcription. The targeted acetylation of histones H3 and H4 at the GAPDH and 
      CA genes is consistent with a role in transcriptional initiation and implies that 
      transcriptional elongation does not necessarily require hyperacetylation.
FAU - Myers, F A
AU  - Myers FA
AD  - Biophysics Laboratories, Institute of Biomedical and Biomolecular Sciences, 
      Faculty of Science, University of Portsmouth, Portsmouth PO1 2DT, United Kingdom.
FAU - Evans, D R
AU  - Evans DR
FAU - Clayton, A L
AU  - Clayton AL
FAU - Thorne, A W
AU  - Thorne AW
FAU - Crane-Robinson, C
AU  - Crane-Robinson C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20010326
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA Primers)
RN  - 0 (Histones)
RN  - 9004-22-2 (Globins)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
RN  - EC 3.1.31.1 (Micrococcal Nuclease)
RN  - EC 4.2.1.1 (Carbonic Anhydrases)
SB  - IM
MH  - Acetylation
MH  - Animals
MH  - Base Sequence
MH  - Carbonic Anhydrases/genetics
MH  - Chick Embryo
MH  - DNA Primers
MH  - Enhancer Elements, Genetic
MH  - Erythrocytes/*enzymology
MH  - Globins/genetics
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/genetics
MH  - Histones/*metabolism
MH  - Micrococcal Nuclease/metabolism
MH  - Transcription, Genetic
EDAT- 2001/03/29 10:00
MHDA- 2001/07/13 10:01
CRDT- 2001/03/29 10:00
PHST- 2001/03/29 10:00 [pubmed]
PHST- 2001/07/13 10:01 [medline]
PHST- 2001/03/29 10:00 [entrez]
AID - S0021-9258(19)40462-6 [pii]
AID - 10.1074/jbc.M009472200 [doi]
PST - ppublish
SO  - J Biol Chem. 2001 Jun 8;276(23):20197-205. doi: 10.1074/jbc.M009472200. Epub 2001 
      Mar 26.