PMID- 11274724
OWN - NLM
STAT- MEDLINE
DCOM- 20010705
LR  - 20190826
IS  - 0165-2478 (Print)
IS  - 0165-2478 (Linking)
VI  - 76
IP  - 2
DP  - 2001 Mar 1
TI  - The response of human dendritic cells to recombinant adenovirus, recombinant 
      Mycobacterium bovis Bacillus Calmette Guerin and biolistic methods of antigen 
      delivery: different induction of contact-dependant and soluble signals.
PG  - 79-88
AB  - Dendritic cells (DCs) are the most potent antigen presenting cells for inducing 
      T-cell immune responses. The ability to grow human DCs from monocyte precursors 
      provides an abundant source of these cells, which can be modified in vitro to 
      present antigens. Re-administration of modified DCs to patients as vaccines has 
      been shown in some cases to induce immune responses against cancer and infectious 
      disease. Gene delivery to DCs provides an intracellular source of antigen for 
      efficient and persistent loading of major histocompatibility complex (MHC) class 
      I molecules. The aim of this study was to use monocyte-derived DCs (MD-DCs) from 
      healthy donors to compare in vitro gene transfer, mediated by adenovirus, M. 
      bovis Bacillus Calmette Guerin (BCG) and biolistic delivery. Efficiency of 
      transfection and effect on DC phenotype, allostimulatory capacity and cytokine 
      secretion was investigated. Adenovirus and BCG both showed a comparable ability 
      to transfect MD-DCs, whereas the biolistic delivery by gene gun was unsuccessful 
      in the reporter gene delivery. BCG transfection promoted MD-DC maturation as is 
      apparent in the surface phenotype, allostimulatory capacity and cytokine 
      secretion from cells. In comparison, adenovirus and biolistic delivery had a 
      reduced effect on MD-DCs although enhancement of co-stimulatory and MHC molecule 
      expression occurred in the cells of some donors. Both BCG and adenovirus 
      represent useful vectors for gene transfer to human DCs. The effect of BCG on DC 
      maturation may provide additional signals for the induction of antigen-specific 
      T-cell responses.
FAU - Smith, S G
AU  - Smith SG
AD  - Applied Immunology Group, ICRF Cancer Medicine Research Unit, St. James's 
      University Hospital, LS7 9TF, Leeds, UK.
FAU - Patel, P M
AU  - Patel PM
FAU - Selby, P J
AU  - Selby PJ
FAU - Jackson, A M
AU  - Jackson AM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Cytokines)
RN  - 0 (Luminescent Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - *Adenoviruses, Human
MH  - Antigen Presentation/*immunology
MH  - Cell Division
MH  - Cell Line
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Dendritic Cells/cytology/*immunology/metabolism
MH  - *Gene Transfer Techniques
MH  - Genes, Reporter
MH  - *Genetic Vectors
MH  - Green Fluorescent Proteins
MH  - Humans
MH  - Luminescent Proteins/genetics
MH  - Monocytes/cytology/metabolism
MH  - *Mycobacterium bovis
MH  - Phenotype
MH  - Recombination, Genetic
MH  - Solubility
MH  - Transfection
EDAT- 2001/03/29 10:00
MHDA- 2001/07/06 10:01
CRDT- 2001/03/29 10:00
PHST- 2001/03/29 10:00 [pubmed]
PHST- 2001/07/06 10:01 [medline]
PHST- 2001/03/29 10:00 [entrez]
AID - S0165247800003242 [pii]
AID - 10.1016/s0165-2478(00)00324-2 [doi]
PST - ppublish
SO  - Immunol Lett. 2001 Mar 1;76(2):79-88. doi: 10.1016/s0165-2478(00)00324-2.