PMID- 11274736
OWN - NLM
STAT- MEDLINE
DCOM- 20010628
LR  - 20190915
IS  - 0168-0102 (Print)
IS  - 0168-0102 (Linking)
VI  - 39
IP  - 4
DP  - 2001 Apr
TI  - Ultrastructural localization of brain-derived neurotrophic factor in rat primary 
      sensory neurons.
PG  - 377-84
AB  - In a previous study we have shown that brain-derived neurotrophic factor (BDNF) 
      is present in a subpopulation of small- to medium-sized sensory neurons in the 
      dorsal root ganglia (DRG) and is anterogradely transported in both the peripheral 
      and central processes. Within the spinal cord, BDNF is localized to varicosities 
      of sensory nerve terminals in laminae I and II of the dorsal horn. This study 
      raised the question of whether BDNF is localized in synaptic vesicles of the 
      afferent nerve terminals. Using immunohistochemical and immunocytochemical 
      techniques we have now investigated the ultrastructural localization of BDNF in 
      the spinal cord of the rat. In addition, its colocalization with the low affinity 
      neurotrophin receptor, p75, and calcitonin gene related peptide (CGRP) was also 
      investigated. In lamina II of the spinal cord, BDNF immunoreactivity was 
      restricted to nerve terminals. The reaction product appeared associated with 
      dense-cored and clear vesicles of terminals superficial laminae. Double labelling 
      experiments at the light microscopic level showed that 55% of BDNF immunoreactive 
      neurons in DRG are colocalized with CGRP and many nerve terminals in laminae I 
      and II of the spinal cord contained both BDNF and CGRP immunoreactivities. The 
      results of double labelling at the ultrastructural level showed that most BDNF-ir 
      (immunoreactive) nerve terminals contained CGRP or the low affinity neurotrophin 
      receptor, p75, but not vice versa. These results point to the conclusion that 
      BDNF may be released in parallel with neurotransmitters from nerve terminals in 
      the spinal cord from a subpopulation of nociceptive primary afferents.
FAU - Luo, X G
AU  - Luo XG
AD  - Department of Anatomy, Human Medical University, Changsha, Hunan, People's 
      Republic of China.
FAU - Rush, R A
AU  - Rush RA
FAU - Zhou, X F
AU  - Zhou XF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Neurosci Res
JT  - Neuroscience research
JID - 8500749
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Fixatives)
RN  - 0 (Receptor, Nerve Growth Factor)
RN  - JHB2QIZ69Z (Calcitonin Gene-Related Peptide)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Calcitonin Gene-Related Peptide/metabolism
MH  - Female
MH  - Fixatives/chemistry
MH  - Ganglia, Spinal/*metabolism/ultrastructure
MH  - Immunohistochemistry
MH  - Male
MH  - Microscopy, Electron
MH  - Neurons, Afferent/*metabolism/ultrastructure
MH  - Posterior Horn Cells/*metabolism/ultrastructure
MH  - Presynaptic Terminals/*metabolism/ultrastructure
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, Nerve Growth Factor/metabolism
EDAT- 2001/03/29 10:00
MHDA- 2001/06/29 10:01
CRDT- 2001/03/29 10:00
PHST- 2001/03/29 10:00 [pubmed]
PHST- 2001/06/29 10:01 [medline]
PHST- 2001/03/29 10:00 [entrez]
AID - S0168010200002388 [pii]
AID - 10.1016/s0168-0102(00)00238-8 [doi]
PST - ppublish
SO  - Neurosci Res. 2001 Apr;39(4):377-84. doi: 10.1016/s0168-0102(00)00238-8.