PMID- 11274811
OWN - NLM
STAT- MEDLINE
DCOM- 20010517
LR  - 20190910
IS  - 0378-5173 (Print)
IS  - 0378-5173 (Linking)
VI  - 216
IP  - 1-2
DP  - 2001 Mar 23
TI  - Flow-through UV spectrophotometric sensor for determination of (acetyl)salicylic 
      acid in pharmaceutical preparations.
PG  - 95-104
AB  - The solid phase spectrophotometry technique, in which the absorbance of the 
      species of interest sorbed on a solid support is measured directly, was applied 
      to the determination of salicylic acid using flow injection-analysis. Salicylic 
      acid was determined by monitoring of its intrinsic absorbance at 297 nm sorbed on 
      Sephadex QAE A-25 resin placed in an appropriate flow-through cell. The method 
      proposed improves the selectivity compared with the corresponding solution-phase 
      method and the sensitivity is increased by a factor of 30 or more. The 
      flow-through sensor proposed allows working with several calibration lines simply 
      by varying the sample volume injected. Thus, linear dynamic ranges from 1 to 20 
      and from 2 to 40 microg ml(-1) can be obtained by using 1000 and 300 microl, 
      respectively, with detection limits being 0.064 and 0.135 microg ml(-1). Relative 
      Standard Deviations (RSDs) of 0.52 and 0.38%, and sampling frequencies of 18 and 
      25 h(-1), respectively, were also achieved. The sensor also allows the indirect 
      determination of acetylsalicylic acid previous hydrolysis on-line to salicylic 
      acid. For acetylsalicylic acid, a linear dynamic range from 5 to 120 microg 
      ml(-1) and 25 h(-1) of sampling frequency (300 microl of sample volume) were 
      obtained. The proposed flow-through sensor has been successfully applied to the 
      determination of both analytes in pharmaceutical preparations.
FAU - Ruiz-Medina, A
AU  - Ruiz-Medina A
AD  - Department of Physical and Analytical Chemistry, Faculty of Experimental 
      Sciences, University of Jaen, Jaen 23071, Spain.
FAU - Fernandez-de Cordova, M L
AU  - Fernandez-de Cordova ML
FAU - Ortega-Barrales, P
AU  - Ortega-Barrales P
FAU - Molina-Diaz, A
AU  - Molina-Diaz A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Int J Pharm
JT  - International journal of pharmaceutics
JID - 7804127
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Pharmaceutical Preparations)
RN  - R16CO5Y76E (Aspirin)
SB  - IM
MH  - Anti-Inflammatory Agents, Non-Steroidal/*analysis
MH  - Aspirin/*analysis
MH  - Flow Injection Analysis/*instrumentation
MH  - Hydrolysis
MH  - Pharmaceutical Preparations/*analysis
MH  - Spectrophotometry, Ultraviolet/*methods
EDAT- 2001/03/29 10:00
MHDA- 2001/05/18 10:01
CRDT- 2001/03/29 10:00
PHST- 2001/03/29 10:00 [pubmed]
PHST- 2001/05/18 10:01 [medline]
PHST- 2001/03/29 10:00 [entrez]
AID - S0378-5173(01)00569-5 [pii]
AID - 10.1016/s0378-5173(01)00569-5 [doi]
PST - ppublish
SO  - Int J Pharm. 2001 Mar 23;216(1-2):95-104. doi: 10.1016/s0378-5173(01)00569-5.