PMID- 11275557
OWN - NLM
STAT- MEDLINE
DCOM- 20010830
LR  - 20190513
IS  - 0021-924X (Print)
IS  - 0021-924X (Linking)
VI  - 129
IP  - 4
DP  - 2001 Apr
TI  - Peptide mimics of monocyte chemoattractant protein-1 (MCP-1) with an antagonistic 
      activity.
PG  - 577-83
AB  - In this study, we attempted to analyze the peptide motifs recognized by 24822.111 
      and F9, monoclonal antibodies (mAbs) that inhibit the chemotactic activity of 
      monocyte chemoattractant protein-1 (MCP-1), a member of the CC subfamily of 
      chemokines. We isolated phage clones from a phage display library and identified 
      six peptide motifs. One of these clones, C27, was strongly and specifically 
      recognized by 24822.111 mAb, while another, G25, was similarly recognized by F9 
      mAb. Both the C27 motif and the G25 motif contain two cysteines in their 
      sequences and have little homology to the primary amino acid sequence of MCP-1. 
      These clones, however, bound to THP-1 cells, and the binding was competitively 
      inhibited by MCP-1. The clones strongly inhibited the MCP-1-induced chemotaxis of 
      human monocytes. The synthetic and intramolecularly disulfide-linked peptides of 
      C27 and G25 (sC27 and sG25) also inhibited the chemotaxis induced by MCP-1, while 
      their derivatives with serine in place of cysteine did not, suggesting the 
      importance of the loop structure for the inhibition. These results suggest that 
      sC27 and sG25 may mimic the MCP-1-binding domain to the MCP-1 receptor.
FAU - Kaji, M
AU  - Kaji M
AD  - Department of Bioengineering, Faculty of Engineering, Kagoshima University, 
      Korimoto, Kagoshima 890-0065, Japan.
FAU - Ikari, M
AU  - Ikari M
FAU - Hashiguchi, S
AU  - Hashiguchi S
FAU - Ito, Y
AU  - Ito Y
FAU - Matsumoto, R
AU  - Matsumoto R
FAU - Yoshimura, T
AU  - Yoshimura T
FAU - Kuratsu Ji
AU  - Kuratsu Ji
FAU - Sugimura, K
AU  - Sugimura K
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Biochem
JT  - Journal of biochemistry
JID - 0376600
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (CCR2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Epitopes)
RN  - 0 (Peptide Library)
RN  - 0 (Peptides)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antibodies, Monoclonal/immunology
MH  - Bacteriophages/genetics/metabolism
MH  - Binding Sites
MH  - Blotting, Western
MH  - Cell Line
MH  - Chemokine CCL2/*antagonists & inhibitors/*chemistry/immunology/pharmacology
MH  - Chemotaxis/drug effects
MH  - Drug Design
MH  - Epitopes/chemistry/immunology
MH  - Flow Cytometry
MH  - Humans
MH  - *Molecular Mimicry
MH  - Peptide Library
MH  - Peptides/*chemistry/immunology/metabolism/*pharmacology
MH  - Protein Binding
MH  - Protein Structure, Tertiary
MH  - Receptors, CCR2
MH  - Receptors, Chemokine/metabolism
EDAT- 2001/03/29 10:00
MHDA- 2001/08/31 10:01
CRDT- 2001/03/29 10:00
PHST- 2001/03/29 10:00 [pubmed]
PHST- 2001/08/31 10:01 [medline]
PHST- 2001/03/29 10:00 [entrez]
AID - 10.1093/oxfordjournals.jbchem.a002893 [doi]
PST - ppublish
SO  - J Biochem. 2001 Apr;129(4):577-83. doi: 10.1093/oxfordjournals.jbchem.a002893.