PMID- 11276045
OWN - NLM
STAT- MEDLINE
DCOM- 20010712
LR  - 20171116
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 64
IP  - 1
DP  - 2001 Apr 1
TI  - Biological characterization and optical imaging of brain-derived neurotrophic 
      factor-green fluorescent protein suggest an activity-dependent local release of 
      brain-derived neurotrophic factor in neurites of cultured hippocampal neurons.
PG  - 1-10
AB  - To visualize the release dynamics of the brain-derived neurotrophic factor (BDNF) 
      involved in neural plasticity, we constructed a plasmid encoding green 
      fluorescent protein (GFP) fused with BDNF. First, several biological studies 
      confirmed that this fusion protein (BDNF-GFP) mimics the biological functions and 
      the release kinetics of unfused (native) BDNF. Second, when BDNF-GFP was 
      expressed in cultured hippocampal neurons, we observed that this protein formed 
      striking clusters in the neurites of mature neurons and colocalized with the 
      PSD-95 immunoreactivity. Such a clustered BDNF-GFP rapidly disappeared in 
      response to depolarization with KCl, as revealed by confocal microscopic studies. 
      These data suggest that BDNF is locally and rapidly released at synaptic sites in 
      an activity-dependent manner. Optical studies using BDNF-GFP may provide 
      important evidence regarding the participation of BDNF in synaptic plasticity.
CI  - Copyright 2001 Wiley-Liss, Inc.
FAU - Kojima, M
AU  - Kojima M
AD  - Precursory Research for Embryonic Science and Technology, Japan Science and 
      Technology Cooperation, Kawaguchi, Saitama, Japan. mkojima@onri.go.jp
FAU - Takei, N
AU  - Takei N
FAU - Numakawa, T
AU  - Numakawa T
FAU - Ishikawa, Y
AU  - Ishikawa Y
FAU - Suzuki, S
AU  - Suzuki S
FAU - Matsumoto, T
AU  - Matsumoto T
FAU - Katoh-Semba, R
AU  - Katoh-Semba R
FAU - Nawa, H
AU  - Nawa H
FAU - Hatanaka, H
AU  - Hatanaka H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Disks Large Homolog 4 Protein)
RN  - 0 (Dlg4 protein, rat)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (postsynaptic density proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 660YQ98I10 (Potassium Chloride)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - Cells, Cultured
MH  - Disks Large Homolog 4 Protein
MH  - Female
MH  - Green Fluorescent Proteins
MH  - Hippocampus/cytology/*metabolism
MH  - Intracellular Signaling Peptides and Proteins
MH  - Kinetics
MH  - Luminescent Proteins/*genetics
MH  - Male
MH  - Membrane Proteins
MH  - Microscopy, Confocal
MH  - Nerve Tissue Proteins/metabolism
MH  - Neurites/*metabolism
MH  - Neuronal Plasticity/physiology
MH  - Neurons/*metabolism
MH  - Potassium Chloride/pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Recombinant Fusion Proteins/*metabolism
MH  - Tissue Distribution
EDAT- 2001/03/29 10:00
MHDA- 2001/07/13 10:01
CRDT- 2001/03/29 10:00
PHST- 2001/03/29 10:00 [pubmed]
PHST- 2001/07/13 10:01 [medline]
PHST- 2001/03/29 10:00 [entrez]
AID - 10.1002/jnr.1080 [doi]
PST - ppublish
SO  - J Neurosci Res. 2001 Apr 1;64(1):1-10. doi: 10.1002/jnr.1080.