PMID- 11276581
OWN - NLM
STAT- MEDLINE
DCOM- 20010524
LR  - 20031114
IS  - 0047-1917 (Print)
IS  - 0047-1917 (Linking)
VI  - 48
IP  - 4
DP  - 2001 Feb
TI  - Relationship among growth, steroid production and immunolocalization of 
      transforming growth factor-beta 1 in the normally developing mouse follicles 
      cultured in vitro.
PG  - 169-76
AB  - This study examined the relationship among growth, steroid production and 
      transforming growth factor-beta 1 (TGF-beta 1) immunolocalization in the mouse 
      follicles cultured in vitro to evaluate the hypothesis that normally developing 
      follicles should express TGF-beta 1 in the granulosa cells around the time of 
      antrum formation. Preantral follicles with 151-175 microns (large category) and 
      125-150 microns (small category) of initial diameters were used as models for 
      normal and retarded follicles, respectively. Growth rate and timing of antrum 
      formation in both categories were comparable to those of in-vivo grown follicles. 
      At the time of antrum formation, follicular diameters were similar between the 
      two follicle categories; however, antral follicles from the large category showed 
      larger number of granulosa cells, higher estradiol production and proportion of 
      follicles with TGF-beta 1 positive granulosa cells. Two days after antrum 
      formation, there were no differences in the number of granulosa cells and the 
      proportions of follicles with TGF-beta 1 positive granulosa or theca cells 
      between the two categories. Temporal association in large follicles between the 
      increase in estradiol production and proportion of follicles with TGF-beta 1 
      positive granulosa cells at the time of antrum formation supports our hypothesis. 
      Furthermore, this study demonstrated the usefulness of the follicle culture 
      system in the investigations of follicular physiology.
FAU - Bishonga, C
AU  - Bishonga C
AD  - Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, 
      Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, 
      Japan.
FAU - Takahashi, Y
AU  - Takahashi Y
FAU - Katagiri, S
AU  - Katagiri S
FAU - Nagano, M
AU  - Nagano M
FAU - Ishikawa, A
AU  - Ishikawa A
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Jpn J Vet Res
JT  - The Japanese journal of veterinary research
JID - 0376567
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Female
MH  - Mice/*growth & development
MH  - Ovarian Follicle/*chemistry
MH  - Transforming Growth Factor beta/*analysis
EDAT- 2001/03/30 10:00
MHDA- 2001/05/26 10:01
CRDT- 2001/03/30 10:00
PHST- 2001/03/30 10:00 [pubmed]
PHST- 2001/05/26 10:01 [medline]
PHST- 2001/03/30 10:00 [entrez]
PST - ppublish
SO  - Jpn J Vet Res. 2001 Feb;48(4):169-76.