PMID- 11277391
OWN - NLM
STAT- MEDLINE
DCOM- 20010510
LR  - 20191104
IS  - 1052-9551 (Print)
IS  - 1052-9551 (Linking)
VI  - 10
IP  - 1
DP  - 2001 Mar
TI  - Translocation (10;11;22)(p14;q24;q12) characterized by fluorescence in situ 
      hybridization in a case of Ewing's tumor.
PG  - 2-8
AB  - It is well recognized that the identification by classic cytogenetics of 
      t(11;22)(q24;q12) is a useful aid in the accurate diagnosis of Ewing's sarcoma 
      and related tumors. This translocation induces the EWS/FLI-1 fusion transcript, 
      which can be detected by reverse transcription-polymerase chain reaction. Recent 
      studies have also used fluorescence in situ hybridization (FISH) to demonstrate 
      the translocation. The authors coupled classic cytogenetics and FISH on tumor 
      cells from the original specimen, the local recurrence, and the pulmonary 
      metastasis as well as from the xenografted tumors in a case of extraosseous 
      Ewing's sarcoma. FISH analysis not only confirmed the cytogenetic results but 
      also allowed the identification of a tumor-specific chromosome change, consistent 
      with a complex translocation, t(10;11;22), as well as revealed other chromosomal 
      rearrangements on both metaphases and interphase nuclei of each material. In 
      addition this technique served to identify, in the interphase nuclei of the 
      original tumor, the clone that became dominant, from the cytogenetic point of 
      view, in the lung metastasis and in the nude mice xenografted tumors. Current 
      results indicate that the use of FISH on metaphases and interphase nuclei is an 
      easy and reliable approach to complement or even to substitute classic 
      cytogenetic studies for the detection of specific chromosomal rearrangements, 
      especially for determining complex translocations and for describing tumoral 
      clones with different cytogenetic markers.
FAU - Noguera, R
AU  - Noguera R
AD  - Department of Pathology, Medical School, University of Valencia, Spain. 
      Rosa.Noguera@uv.es
FAU - Pellin, A
AU  - Pellin A
FAU - Navarro, S
AU  - Navarro S
FAU - Carda, C
AU  - Carda C
FAU - Llombart-Bosch, A
AU  - Llombart-Bosch A
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Diagn Mol Pathol
JT  - Diagnostic molecular pathology : the American journal of surgical pathology, part 
      B
JID - 9204924
RN  - 0 (DNA, Neoplasm)
RN  - 0 (EWS-FLI fusion protein)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (Proto-Oncogene Protein c-fli-1)
RN  - 0 (RNA-Binding Protein EWS)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Bone Neoplasms/*genetics/pathology/therapy
MH  - Child
MH  - Chromosomes, Human, Pair 10/*genetics
MH  - Chromosomes, Human, Pair 11/*genetics
MH  - Chromosomes, Human, Pair 22/*genetics
MH  - Combined Modality Therapy
MH  - DNA, Neoplasm/analysis
MH  - Fatal Outcome
MH  - Female
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leg/pathology
MH  - Lung Neoplasms/secondary
MH  - Oncogene Proteins, Fusion/analysis
MH  - Proto-Oncogene Protein c-fli-1
MH  - RNA-Binding Protein EWS
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sarcoma, Ewing/*genetics/secondary/therapy
MH  - Sequence Analysis, DNA
MH  - Transcription Factors/analysis
MH  - *Translocation, Genetic
EDAT- 2001/03/30 10:00
MHDA- 2001/05/22 10:01
CRDT- 2001/03/30 10:00
PHST- 2001/03/30 10:00 [pubmed]
PHST- 2001/05/22 10:01 [medline]
PHST- 2001/03/30 10:00 [entrez]
AID - 10.1097/00019606-200103000-00002 [doi]
PST - ppublish
SO  - Diagn Mol Pathol. 2001 Mar;10(1):2-8. doi: 10.1097/00019606-200103000-00002.