PMID- 11277872
OWN - NLM
STAT- MEDLINE
DCOM- 20010719
LR  - 20101118
IS  - 1470-1626 (Print)
IS  - 1470-1626 (Linking)
VI  - 121
IP  - 4
DP  - 2001 Apr
TI  - An X-Y paint set and sperm FISH protocol that can be used for validation of 
      cattle sperm separation procedures.
PG  - 541-6
AB  - X and Y chromosome paints were developed from sorted yak chromosomes for sexing 
      cattle spermatozoa. Clear hybridization signals were obtained for every 
      spermatozoon using a modified sperm decondensation protocol and fluorescence in 
      situ hybridization (FISH). The procedure was evaluated using the established 
      Beltsville sperm sexing technology, which separates spermatozoa by flow cytometry 
      into X- and Y-bearing fractions. Close agreement was found between the assessment 
      of sperm separation by flow cytometry and by FISH with the X-Y paint set. The 
      FISH method is a simple, reliable and robust procedure for assessing the 
      effectiveness of separation of X and Y spermatozoa.
FAU - Rens, W
AU  - Rens W
AD  - Centre for Veterinary Science, Department of Clinical Veterinary Medicine, 
      Veterinary Cytogenetics Group, University of Cambridge, Madingley Road, Cambridge 
      CB3 0ES, UK. wr203@mole.bio.cam.ac.uk
FAU - Yang, F
AU  - Yang F
FAU - Welch, G
AU  - Welch G
FAU - Revell, S
AU  - Revell S
FAU - O'Brien, P C
AU  - O'Brien PC
FAU - Solanky, N
AU  - Solanky N
FAU - Johnson, L A
AU  - Johnson LA
FAU - Ferguson Smith, M A
AU  - Ferguson Smith MA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Reproduction
JT  - Reproduction (Cambridge, England)
JID - 100966036
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cell Separation/*methods
MH  - Cells, Cultured
MH  - *Chromosome Painting
MH  - DNA/analysis/chemistry
MH  - Flow Cytometry
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Sex Determination Analysis
MH  - Spermatozoa/*cytology/ultrastructure
MH  - *X Chromosome
MH  - *Y Chromosome
EDAT- 2001/03/30 10:00
MHDA- 2001/07/20 10:01
CRDT- 2001/03/30 10:00
PHST- 2001/03/30 10:00 [pubmed]
PHST- 2001/07/20 10:01 [medline]
PHST- 2001/03/30 10:00 [entrez]
PST - ppublish
SO  - Reproduction. 2001 Apr;121(4):541-6.