PMID- 11277995
OWN - NLM
STAT- MEDLINE
DCOM- 20010426
LR  - 20220310
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 493
IP  - 1
DP  - 2001 Mar 23
TI  - TNF-alpha inhibits UCP-1 expression in brown adipocytes via ERKs. Opposite effect 
      of p38MAPK.
PG  - 6-11
AB  - Tumor necrosis factor-alpha (TNF-alpha) activates extracellular-regulated kinases 
      (ERKs) and p38 mitogen-activated protein kinase (p38MAPK), and inhibits the 
      expression of uncoupling protein-1 (UCP-1) and adipocyte-specific genes in rat 
      fetal brown adipocytes. MEK inhibition with PD98059 abolished the inhibitory 
      effect of TNF-alpha on UCP-1, but not on adipogenic genes. In contrast, 
      inhibition of p38MAPK with SB203580 potentiated the negative effect of TNF-alpha 
      on UCP-1 and adipogenic genes. The inhibitory action of TNF-alpha was partially 
      correlated with changes in C/EBPalpha and beta protein levels and in their DNA 
      binding activity, suggesting a role for these transcription factors. However, 
      other transcription factors might explain the different regulation of UCP-1 and 
      adipogenic genes by ERKs.
FAU - Valladares, A
AU  - Valladares A
AD  - Departamento de Bioquimica y Biologia Molecular II, Instituto de Bioquimica 
      (Centro Mixto del Consejo Superior de Investigaciones Cientificas (C.S.I.C.) y de 
      la Universidad Complutense de Madrid (U.C.M.)), Madrid, Spain.
FAU - Roncero, C
AU  - Roncero C
FAU - Benito, M
AU  - Benito M
FAU - Porras, A
AU  - Porras A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (CCAAT-Enhancer-Binding Protein-alpha)
RN  - 0 (CCAAT-Enhancer-Binding Protein-beta)
RN  - 0 (Carrier Proteins)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Flavonoids)
RN  - 0 (Imidazoles)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Ion Channels)
RN  - 0 (Membrane Proteins)
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (Pyridines)
RN  - 0 (Transcription Factors)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Ucp1 protein, rat)
RN  - 0 (Uncoupling Protein 1)
RN  - 63231-63-0 (RNA)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - EC 2.7.1.- (MAP-kinase-activated kinase 2)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
RN  - OU13V1EYWQ (SB 203580)
RN  - SJE1IO5E3I (2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one)
SB  - IM
MH  - Adipocytes/*metabolism
MH  - Adipose Tissue, Brown/embryology/*metabolism
MH  - Animals
MH  - Apoptosis
MH  - Blotting, Northern
MH  - Blotting, Western
MH  - CCAAT-Enhancer-Binding Protein-alpha/metabolism
MH  - CCAAT-Enhancer-Binding Protein-beta/metabolism
MH  - Carrier Proteins/*biosynthesis
MH  - Cell Differentiation
MH  - Cell Nucleus/metabolism
MH  - Cells, Cultured
MH  - Enzyme Inhibitors/pharmacology
MH  - Flavonoids/pharmacology
MH  - Imidazoles/pharmacology
MH  - Insulin-Like Growth Factor I/metabolism
MH  - Intracellular Signaling Peptides and Proteins
MH  - Ion Channels
MH  - Membrane Proteins/*biosynthesis
MH  - Mitochondrial Proteins
MH  - Mitogen-Activated Protein Kinase Kinases/metabolism
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - Protein Binding
MH  - Protein Serine-Threonine Kinases/metabolism
MH  - Pyridines/pharmacology
MH  - RNA/metabolism
MH  - Rats
MH  - Transcription Factors/metabolism
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - Uncoupling Protein 1
MH  - p38 Mitogen-Activated Protein Kinases
EDAT- 2001/03/30 10:00
MHDA- 2001/05/01 10:01
CRDT- 2001/03/30 10:00
PHST- 2001/03/30 10:00 [pubmed]
PHST- 2001/05/01 10:01 [medline]
PHST- 2001/03/30 10:00 [entrez]
AID - S0014-5793(01)02264-5 [pii]
AID - 10.1016/s0014-5793(01)02264-5 [doi]
PST - ppublish
SO  - FEBS Lett. 2001 Mar 23;493(1):6-11. doi: 10.1016/s0014-5793(01)02264-5.