PMID- 11279020
OWN - NLM
STAT- MEDLINE
DCOM- 20010705
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 276
IP  - 21
DP  - 2001 May 25
TI  - Profiling changes in gene expression during differentiation and maturation of 
      monocyte-derived dendritic cells using both oligonucleotide microarrays and 
      proteomics.
PG  - 17920-31
AB  - Dendritic cells (DCs) are antigen-presenting cells that play a major role in 
      initiating primary immune responses. We have utilized two independent approaches, 
      DNA microarrays and proteomics, to analyze the expression profile of human 
      CD14(+) blood monocytes and their derived DCs. Analysis of gene expression 
      changes at the RNA level using oligonucleotide microarrays complementary to 6300 
      human genes showed that approximately 40% of the genes were expressed in DCs. A 
      total of 255 genes (4%) were found to be regulated during DC differentiation or 
      maturation. Most of these genes were not previously associated with DCs and 
      included genes encoding secreted proteins as well as genes involved in cell 
      adhesion, signaling, and lipid metabolism. Protein analysis of the same cell 
      populations was done using two-dimensional gel electrophoresis. A total of 900 
      distinct protein spots were included, and 4% of them exhibited quantitative 
      changes during DC differentiation and maturation. Differentially expressed 
      proteins were identified by mass spectrometry and found to represent proteins 
      with Ca(2+) binding, fatty acid binding, or chaperone activities as well as 
      proteins involved in cell motility. In addition, proteomic analysis provided an 
      assessment of post-translational modifications. The chaperone protein, 
      calreticulin, was found to undergo cleavage, yielding a novel form. The combined 
      oligonucleotide microarray and proteomic approaches have uncovered novel genes 
      associated with DC differentiation and maturation and has allowed analysis of 
      post-translational modifications of specific proteins as part of these processes.
FAU - Le Naour, F
AU  - Le Naour F
AD  - Department of Microbiology, University of Michigan, Ann Arbor, Michigan 
      48109-0666, USA.
FAU - Hohenkirk, L
AU  - Hohenkirk L
FAU - Grolleau, A
AU  - Grolleau A
FAU - Misek, D E
AU  - Misek DE
FAU - Lescure, P
AU  - Lescure P
FAU - Geiger, J D
AU  - Geiger JD
FAU - Hanash, S
AU  - Hanash S
FAU - Beretta, L
AU  - Beretta L
LA  - eng
PT  - Journal Article
DEP - 20010308
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Proteome)
SB  - IM
MH  - Amino Acid Sequence
MH  - Cell Differentiation/genetics
MH  - Dendritic Cells/cytology/*physiology
MH  - *Gene Expression Profiling
MH  - Gene Expression Regulation
MH  - Humans
MH  - Molecular Sequence Data
MH  - Monocytes/cytology/physiology
MH  - Oligonucleotide Array Sequence Analysis
MH  - Proteome
EDAT- 2001/03/30 10:00
MHDA- 2001/07/06 10:01
CRDT- 2001/03/30 10:00
PHST- 2001/03/30 10:00 [pubmed]
PHST- 2001/07/06 10:01 [medline]
PHST- 2001/03/30 10:00 [entrez]
AID - S0021-9258(19)31726-0 [pii]
AID - 10.1074/jbc.M100156200 [doi]
PST - ppublish
SO  - J Biol Chem. 2001 May 25;276(21):17920-31. doi: 10.1074/jbc.M100156200. Epub 2001 
      Mar 8.