PMID- 11279196
OWN - NLM
STAT- MEDLINE
DCOM- 20010726
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 276
IP  - 22
DP  - 2001 Jun 1
TI  - A novel p75TNF receptor isoform mediating NFkappa B activation.
PG  - 19390-5
AB  - We report the identification of a novel p75TNF receptor isoform termed icp75TNFR, 
      which is generated by the use of an alternative transcriptional start site within 
      the p75TNFR gene and characterized by regulated intracellular expression. The 
      icp75TNFR protein has an apparent molecular mass of approximately 50 kDa and is 
      recognized by antibodies generated against the transmembrane form of p75TNFR. The 
      icp75TNFR binds the tumor necrosis factor(TNF) and mediates intracellular 
      signaling. Overexpression of the icp75TNFR cDNA results in TNF-induced activation 
      of NFkappaB in a TNF receptor-associated factor 2 (TRAF2)-dependent manner. Thus, 
      our results provide an example for intracellular cytokine receptor activation.
FAU - Seitz, C
AU  - Seitz C
AD  - Department of Pathology/Tumor Immunology, University of Regensburg, D-93042 
      Regensburg, Germany.
FAU - Muller, P
AU  - Muller P
FAU - Krieg, R C
AU  - Krieg RC
FAU - Mannel, D N
AU  - Mannel DN
FAU - Hehlgans, T
AU  - Hehlgans T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20010301
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Antigens, CD)
RN  - 0 (DNA, Complementary)
RN  - 0 (NF-kappa B)
RN  - 0 (Protein Isoforms)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Receptors, Tumor Necrosis Factor, Type II)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (TNF Receptor-Associated Factor 2)
SB  - IM
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, CD/*chemistry
MH  - Base Sequence
MH  - Blotting, Western
MH  - Cell Line
MH  - DNA, Complementary/metabolism
MH  - Enzyme Activation
MH  - Exons
MH  - Genes, Reporter
MH  - HeLa Cells
MH  - Humans
MH  - Mice
MH  - Microscopy, Fluorescence
MH  - Models, Genetic
MH  - Molecular Sequence Data
MH  - NF-kappa B/*metabolism
MH  - Open Reading Frames
MH  - Protein Binding
MH  - *Protein Isoforms
MH  - Protein Structure, Tertiary
MH  - Proteins/metabolism
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Receptors, Tumor Necrosis Factor/*chemistry
MH  - Receptors, Tumor Necrosis Factor, Type II
MH  - Recombinant Fusion Proteins/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Homology, Amino Acid
MH  - Signal Transduction
MH  - TNF Receptor-Associated Factor 2
MH  - Transcription, Genetic
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - U937 Cells
EDAT- 2001/03/30 10:00
MHDA- 2001/07/28 10:01
CRDT- 2001/03/30 10:00
PHST- 2001/03/30 10:00 [pubmed]
PHST- 2001/07/28 10:01 [medline]
PHST- 2001/03/30 10:00 [entrez]
AID - S0021-9258(19)67081-X [pii]
AID - 10.1074/jbc.M101336200 [doi]
PST - ppublish
SO  - J Biol Chem. 2001 Jun 1;276(22):19390-5. doi: 10.1074/jbc.M101336200. Epub 2001 
      Mar 1.