PMID- 11279263
OWN - NLM
STAT- MEDLINE
DCOM- 20010503
LR  - 20190630
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 77
IP  - 1
DP  - 2001 Apr
TI  - Induction of brain-derived neurotrophic factor by convulsant drugs in the rat 
      brain: involvement of region-specific voltage-dependent calcium channels.
PG  - 71-83
AB  - A high level of hippocampal brain-derived neurotrophic factor (BDNF) in normally 
      aged as compared with young rats suggests that it is important to maintain a 
      considerable level of hippocampal BDNF during aging in order to keep normal 
      hippocampal functions. To elucidate possible mechanisms of endogenous BDNF 
      increase, changes in levels of BDNF were studied in the rat brain following 
      systemic administration of various convulsant agents; excitotoxic glutamate 
      agonists, NMDA, kainic acid and 
      (+/-)-alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA); GABA 
      receptor antagonists, picrotoxin, pentylenetetrazole (PTZ) and lindane 
      (gamma-hexachlorocyclohexane); and L-type voltage-dependent calcium channel 
      agonist, BAY-K 8644. Kainic acid and AMPA, but not NMDA, caused remarkable 
      increases in BDNF protein in the rat hippocampus and entorhinal cortex. 
      Picrotoxin, PTZ and lindane stimulated BDNF production in the entorhinal cortex 
      and also in the hippocampus of rats showing very severe convulsions. On the other 
      hand, BAY-K 8644 treatment increased BDNF levels in the neocortex and entorhinal 
      cortex. Maximal levels of BDNF protein were observed at 12--24 h, 8--16 h and 6 h 
      following administration of kainic acid, PTZ and BAY-K 8644, respectively. Kainic 
      acid stimulated BDNF synthesis in presynaptic hippocampal granule neurons, but 
      not in postsynaptic neurons with its receptors, while PTZ and BAY-K 8644 produced 
      the same effects in postsynaptic neurons in the entorhinal cortex (in granule 
      neurons in the hippocampus) and in the whole cortex, respectively. Nifedipine 
      inhibited almost completely BAY-K 8644, but not PTZ, effects. omega-Conotoxin 
      GVIA and DCG-IV partially blocked kainic acid-induced enhancement of BDNF, 
      indicating involvement of L-type and N-type voltage-dependent calcium channels, 
      respectively. In addition, BDNF levels in the hippocampus of mice deficient in 
      D-myo-inositol-1,4,5-triphosphate receptor gene were scarcely different from 
      those in the same region of controls, suggesting little involvement of 
      intracellular calcium increase through this receptor. BAY-K 8644, but not kainic 
      acid or PTZ, stimulated the phosphorylation of cyclic AMP responsive element 
      binding protein. Our results indicate convulsant-dependent stimulation of BDNF 
      production and involvement of region-specific voltage-dependent calcium channels.
FAU - Katoh-Semba, R
AU  - Katoh-Semba R
AD  - Institute for Developmental Research, Aichi Human Service Center, Aichi, Japan. 
      katohsemba@inst-hsc.pref.aichi.jp
FAU - Takeuchi, I K
AU  - Takeuchi IK
FAU - Inaguma, Y
AU  - Inaguma Y
FAU - Ichisaka, S
AU  - Ichisaka S
FAU - Hata, Y
AU  - Hata Y
FAU - Tsumoto, T
AU  - Tsumoto T
FAU - Iwai, M
AU  - Iwai M
FAU - Mikoshiba, K
AU  - Mikoshiba K
FAU - Kato, K
AU  - Kato K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Calcium Channel Agonists)
RN  - 0 (Calcium Channel Blockers)
RN  - 0 (Calcium Channels)
RN  - 0 (Convulsants)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Excitatory Amino Acid Agonists)
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (Inositol 1,4,5-Trisphosphate Receptors)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
SB  - IM
MH  - Animals
MH  - Brain/*drug effects/*metabolism
MH  - Brain-Derived Neurotrophic Factor/analysis/*metabolism
MH  - Calcium Channel Agonists/pharmacology
MH  - Calcium Channel Blockers/pharmacology
MH  - Calcium Channels/deficiency/genetics/*metabolism
MH  - Convulsants/*pharmacology
MH  - Cyclic AMP Response Element-Binding Protein/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Entorhinal Cortex/drug effects/metabolism
MH  - Excitatory Amino Acid Agonists/pharmacology
MH  - Excitatory Amino Acid Antagonists/pharmacology
MH  - Female
MH  - Hippocampus/drug effects/metabolism
MH  - Inositol 1,4,5-Trisphosphate Receptors
MH  - Male
MH  - Mice
MH  - Mice, Knockout
MH  - Neocortex/drug effects/metabolism
MH  - Organ Specificity/drug effects
MH  - Phosphorylation/drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Cytoplasmic and Nuclear/deficiency/genetics
MH  - Seizures/chemically induced
MH  - Survival Rate
EDAT- 2001/03/30 10:00
MHDA- 2001/05/05 10:01
CRDT- 2001/03/30 10:00
PHST- 2001/03/30 10:00 [pubmed]
PHST- 2001/05/05 10:01 [medline]
PHST- 2001/03/30 10:00 [entrez]
AID - 10.1046/j.1471-4159.2001.t01-1-00138.x [doi]
PST - ppublish
SO  - J Neurochem. 2001 Apr;77(1):71-83. doi: 10.1046/j.1471-4159.2001.t01-1-00138.x.