PMID- 11279298
OWN - NLM
STAT- MEDLINE
DCOM- 20010705
LR  - 20190513
IS  - 1360-9947 (Print)
IS  - 1360-9947 (Linking)
VI  - 7
IP  - 4
DP  - 2001 Apr
TI  - Expression of 11 beta-hydroxysteroid dehydrogenase isozymes and corticosteroid 
      hormone receptors in primary cultures of human trophoblast and placental bed 
      biopsies.
PG  - 357-63
AB  - Interconversion of active and inactive glucocorticoids, e.g. cortisol (F) and 
      cortisone (E) is catalysed by 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD) 
      which exists as two isoforms. We have used human placental bed biopsies and an 
      in-vitro cytotrophoblast cell culture system to examine the expression and 
      activity of the 11 beta-HSD isoforms along with that of the glucocorticoid and 
      mineralocorticoid receptors (GR and MR). Immunohistochemistry localized 11 
      beta-HSD1 to decidualized stromal cells and 11 beta-HSD2 to villous 
      cytotrophoblast, syncytiotrophoblasts and trophoblast cells invading the 
      placental bed and maternal vasculature. In primary cultures of human 
      cytotrophoblast, 11 beta-HSD2, GR and MR mRNA were expressed. Low levels of 11 
      beta-HSD1 mRNA were noted in these cultured cells, but could be explained on the 
      basis of contaminating, vimentin-positive decidual stromal cells (< or =5%). 
      Enzyme activity studies confirmed the presence of a high-affinity, NAD-dependent 
      dehydrogenase activity (K(m) 137 nmol/l and V(max) 128 pmol E/h/mg protein), 
      indicative of the 11 beta-HSD2 isoform. No reductase activity was observed. The 
      presence of functional MR and GR was determined using Scatchard analyses of 
      dexamethasone and aldosterone binding (MR K(d) 1.4 nmol/l B(max) 3.0; GR K(d) 6.6 
      nmol/l B(max) 16.2 fmol/ng protein). The expression of 11 beta-HSD1 in maternal 
      decidua and 11 beta-HSD2 in adjacent trophoblast suggests an important role for 
      glucocorticoids in determining trophoblast invasion. The presence of the MR 
      within trophoblast indicates that some of the effects of cortisol could be MR- 
      rather than GR-mediated.
FAU - Driver, P M
AU  - Driver PM
AD  - Division of Medical Sciences, University of Birmingham, Queen Elizabeth Hospital, 
      Edgbaston, Birmingham B15 2TH, UK.
FAU - Kilby, M D
AU  - Kilby MD
FAU - Bujalska, I
AU  - Bujalska I
FAU - Walker, E A
AU  - Walker EA
FAU - Hewison, M
AU  - Hewison M
FAU - Stewart, P M
AU  - Stewart PM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mol Hum Reprod
JT  - Molecular human reproduction
JID - 9513710
RN  - 0 (Isoenzymes)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (Receptors, Mineralocorticoid)
RN  - EC 1.1.- (Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenase Type 2)
RN  - EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.146 (HSD11B2 protein, human)
SB  - IM
MH  - 11-beta-Hydroxysteroid Dehydrogenase Type 2
MH  - 11-beta-Hydroxysteroid Dehydrogenases
MH  - Cells, Cultured
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Hydroxysteroid Dehydrogenases/genetics/*metabolism
MH  - Isoenzymes/genetics/metabolism
MH  - Kinetics
MH  - Placenta/cytology/*metabolism
MH  - RNA, Messenger
MH  - Receptors, Glucocorticoid/genetics/*metabolism
MH  - Receptors, Mineralocorticoid/genetics/*metabolism
MH  - Trophoblasts/cytology/*metabolism
EDAT- 2001/03/30 10:00
MHDA- 2001/07/06 10:01
CRDT- 2001/03/30 10:00
PHST- 2001/03/30 10:00 [pubmed]
PHST- 2001/07/06 10:01 [medline]
PHST- 2001/03/30 10:00 [entrez]
AID - 10.1093/molehr/7.4.357 [doi]
PST - ppublish
SO  - Mol Hum Reprod. 2001 Apr;7(4):357-63. doi: 10.1093/molehr/7.4.357.