PMID- 11280916
OWN - NLM
STAT- MEDLINE
DCOM- 20010517
LR  - 20071115
IS  - 0485-1439 (Print)
IS  - 0485-1439 (Linking)
VI  - 42
IP  - 2
DP  - 2001 Feb
TI  - [Complex translocation (8;15;21) (q22;p12;q22) in a child with AML-M2 showing de 
      novo appearance of the short form of AML1-MTG8 chimeric mRNA during the course].
PG  - 110-4
AB  - A 4-year-old boy admitted with exophthalmos was diagnosed as having acute 
      myeloblastic leukemia with maturation (AML-M2). Chromosomal analysis (G-banding) 
      showed t(8;15;21). Fluorescence in situ hybridization (FISH), reverse 
      transcriptase-polymerase chain reaction (RT-PCR), spectral karyotyping (SKY), and 
      nucleolar organizer region (NOR) staining suggested that this complex 
      translocation might have resulted from stepwise translocation, namely an initial 
      translocation between chromosomes 8 and 21, followed by a second translocation 
      between der(21) and chromosome 15, rather than the other possibility of clockwise 
      translocation. During chemotherapy, RT-PCR demonstrated the short form of 
      AML1-MTG8 mRNA, in addition to chimeric mRNA of the usual length. Sequence 
      analysis revealed that this shorter chimeric mRNA had resulted from deletion of a 
      250-bp sequence at the 5' end of MTG8. A literature search failed to reveal any 
      similar cases of t(8;21) AML-M2 associated with this deletion of chimeric mRNA.
FAU - Watanabe, A
AU  - Watanabe A
AD  - Department of Pediatrics, Ibaragi Children's Hospital.
FAU - Koike, K
AU  - Koike K
FAU - Fukushima, T
AU  - Fukushima T
FAU - Izumi, I
AU  - Izumi I
FAU - Ohba, K
AU  - Ohba K
FAU - Tsuchida, M
AU  - Tsuchida M
LA  - jpn
PT  - Case Reports
PT  - English Abstract
PT  - Journal Article
PL  - Japan
TA  - Rinsho Ketsueki
JT  - [Rinsho ketsueki] The Japanese journal of clinical hematology
JID - 2984782R
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Base Sequence
MH  - Child, Preschool
MH  - Chromosomes, Human, Pair 15/*genetics
MH  - Chromosomes, Human, Pair 21/*genetics
MH  - Chromosomes, Human, Pair 8/*genetics
MH  - Humans
MH  - Leukemia, Myeloid, Acute/*genetics
MH  - Male
MH  - RNA, Messenger/*genetics
MH  - *Translocation, Genetic
EDAT- 2001/04/03 10:00
MHDA- 2001/05/18 10:01
CRDT- 2001/04/03 10:00
PHST- 2001/04/03 10:00 [pubmed]
PHST- 2001/05/18 10:01 [medline]
PHST- 2001/04/03 10:00 [entrez]
PST - ppublish
SO  - Rinsho Ketsueki. 2001 Feb;42(2):110-4.