PMID- 11282210
OWN - NLM
STAT- MEDLINE
DCOM- 20010621
LR  - 20190728
IS  - 0264-410X (Print)
IS  - 0264-410X (Linking)
VI  - 19
IP  - 20-22
DP  - 2001 Apr 6
TI  - Quantitative determination of C-polysaccharide in Streptococcus pneumoniae 
      capsular polysaccharides by use of high-performance anion-exchange chromatography 
      with pulsed amperometric detection.
PG  - 2987-94
AB  - Capsular polysaccharides of Streptococcus pneumoniae are used to formulate 
      polyvalent pneumococcal vaccines. A sensitive method, using high-performance 
      anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), has 
      been developed to quantify the contamination of pneumococcal capsular 
      polysaccharides (PnPs) with the C-polysaccharide (C-Ps). As this polysaccharide 
      is highly immunogenic, and since anti C-Ps antibodies are not protective, the 
      need to monitor and reduce its level is of uppermost importance. The method is 
      based on the quantification by HPAEC-PAD of ribitol, which is released by a 
      two-step hydrolysis of the PnPs using aqueous hydrofluoric acid (HF) followed by 
      trifluoroacetic acid hydrolysis (TFA). This simple method has been shown to 
      provide both qualitative and quantitative information about the purity of 
      polysaccharide preparations.
FAU - Talaga, P
AU  - Talaga P
AD  - Aventis Pasteur, Campus Merieux, Biochemistry Research Department, 1541 Avenue 
      Marcel Merieux, 69280 Marcy l'Etoile, France. philippe.talaga@aventis.com
FAU - Bellamy, L
AU  - Bellamy L
FAU - Moreau, M
AU  - Moreau M
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
SB  - IM
MH  - Bacterial Capsules/*analysis
MH  - Chromatography, Ion Exchange
MH  - Electrochemistry
MH  - Magnetic Resonance Spectroscopy
MH  - Streptococcus pneumoniae/*chemistry
EDAT- 2001/04/03 10:00
MHDA- 2001/06/22 10:01
CRDT- 2001/04/03 10:00
PHST- 2001/04/03 10:00 [pubmed]
PHST- 2001/06/22 10:01 [medline]
PHST- 2001/04/03 10:00 [entrez]
AID - S0264-410X(00)00535-1 [pii]
AID - 10.1016/s0264-410x(00)00535-1 [doi]
PST - ppublish
SO  - Vaccine. 2001 Apr 6;19(20-22):2987-94. doi: 10.1016/s0264-410x(00)00535-1.