PMID- 11282457
OWN - NLM
STAT- MEDLINE
DCOM- 20010823
LR  - 20190921
IS  - 0898-6568 (Print)
IS  - 0898-6568 (Linking)
VI  - 13
IP  - 3
DP  - 2001 Mar
TI  - Resveratrol inhibits the formation of phosphatidic acid and diglyceride in 
      chemotactic peptide- or phorbol ester-stimulated human neutrophils.
PG  - 191-7
AB  - Resveratrol (trans-3,5,4'-trihydroxystilbene, Res) is a naturally occurring 
      antioxidant found in grape berry skins and red wine. It has anti-inflammatory 
      effects. In this study, we examined the effect of Res on the formation of 
      phosphatidic acid (PA) and diglyceride (DG), in human neutrophils stimulated by 
      formyl-methionyl-leucyl-phenylalanine (fMLP) or by phorbol 12-myristate 
      13-acetate (PMA). We measured the masses of PA and DG by using a nonradioactive 
      method. Our results showed that Res inhibited the formation of PA in a 
      concentration dependent manner with an IC(50) value of 42.4 and 60.9 microM in 
      fMLP- and PMA-stimulated cells, respectively. Res also suppressed the formation 
      of phosphatidylethanol (PEt), thereby implying inhibition of phospholipase D 
      (PLD) activity. In addition, Res inhibited the formation of both diacylglycerol 
      (DAG) and ether-linked acylglycerol (EAG) induced by fMLP and by PMA. Our results 
      suggest that Res inhibition of PLD activity may contribute to its 
      anti-inflammatory effects.
FAU - Tou, J
AU  - Tou J
AD  - Department of Biochemistry, Tulane University Health Sciences Center, SL 43, 1430 
      Tulane Avenue, 70112, New Orleans, LA, USA. jtou@tulane.edu
FAU - Urbizo, C
AU  - Urbizo C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cell Signal
JT  - Cellular signalling
JID - 8904683
RN  - 0 (Diglycerides)
RN  - 0 (Glycerophosphates)
RN  - 0 (Glycerophospholipids)
RN  - 0 (Phosphatidic Acids)
RN  - 0 (Phosphatidylcholines)
RN  - 0 (Stilbenes)
RN  - 0 (phosphatidylethanol)
RN  - 3K9958V90M (Ethanol)
RN  - 57716-89-9 (4-O-methyl-12-O-tetradecanoylphorbol 13-acetate)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 5KV86114PT (Ethanolamine)
RN  - N91BDP6H0X (Choline)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - Q369O8926L (Resveratrol)
SB  - IM
MH  - Choline/metabolism
MH  - Chromatography, Thin Layer
MH  - Diglycerides/*metabolism
MH  - Drug Interactions
MH  - Ethanol/metabolism
MH  - Ethanolamine/metabolism
MH  - Glycerophosphates/metabolism
MH  - Glycerophospholipids/*antagonists & inhibitors
MH  - Humans
MH  - In Vitro Techniques
MH  - N-Formylmethionine Leucyl-Phenylalanine/*pharmacology
MH  - Neutrophils/*drug effects/metabolism
MH  - Phosphatidic Acids/*metabolism
MH  - Phosphatidylcholines/metabolism
MH  - Resveratrol
MH  - Stilbenes/*pharmacology
MH  - Tetradecanoylphorbol Acetate/analogs & derivatives/*pharmacology
MH  - Time Factors
EDAT- 2001/04/03 10:00
MHDA- 2001/08/24 10:01
CRDT- 2001/04/03 10:00
PHST- 2001/04/03 10:00 [pubmed]
PHST- 2001/08/24 10:01 [medline]
PHST- 2001/04/03 10:00 [entrez]
AID - S0898656801001371 [pii]
AID - 10.1016/s0898-6568(01)00137-1 [doi]
PST - ppublish
SO  - Cell Signal. 2001 Mar;13(3):191-7. doi: 10.1016/s0898-6568(01)00137-1.