PMID- 11283070
OWN - NLM
STAT- MEDLINE
DCOM- 20010823
LR  - 20221029
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 39
IP  - 4
DP  - 2001 Apr
TI  - Definition of a divergent epitope that allows differential detection of early 
      protein p41 from human herpesvirus 6 variants A and B.
PG  - 1449-55
AB  - The human herpesvirus 6 (HHV-6) early protein, p41, encoded by the U27 gene has 
      been detected in oligodendrocytes of multiple sclerosis (MS) patients by using a 
      monoclonal antibody (MAb to p41/38). We here report the antigenic epitope of 
      HHV-6 p41 recognized by this MAb. First, we established that the MAb to p41/38 
      recognizes a nuclear antigen in HHV-6A strain GS-infected cells but not in HHV-6B 
      strain Z29-infected cells. Secondly, we compared the reactivity of the MAb to 
      p41/38 to that of another p41-specific MAb (MAb to p41) on immunoblots with 
      purified p41-glutathione S-transferase fusion protein from strains GS and Z29 and 
      GS- and Z29-infected-cell lysates. The two MAbs were tested in an enzyme-linked 
      immunosorbent assay against a panel of synthetic peptides covering the amino acid 
      substitutions between the GS- and Z29-derived p41 proteins, as determined by DNA 
      sequencing of our cloned isolates of the U27 gene. The MAb to p41/38 reacted 
      specifically with a peptide comprising p41 residues 321 to 340 from strain GS. 
      The critical residue in this peptide was serine 328, as the substitution S328N in 
      the Z29 strain rendered the corresponding peptide nonreactive. The p41 S328 
      marker was present in three of three HHV-6A strains, while four of four sequenced 
      p41 genes from HHV-6B strains had N328. Our findings are of value for the 
      interpretation of previous findings of p41 expression in brains of MS patients 
      and may allow a more detailed analysis of the role of HHV-6 variants in other 
      disorders.
FAU - Xu, Y
AU  - Xu Y
AD  - Department of Virology, Swedish Institute for Infectious Disease Control, 171 82 
      Solna, Sweden.
FAU - Linde, A
AU  - Linde A
FAU - Dahl, H
AU  - Dahl H
FAU - Winberg, G
AU  - Winberg G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Viral)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Epitopes)
RN  - 0 (Peptides)
RN  - 0 (Viral Proteins)
RN  - 138415-18-6 (p41 protein, Human herpesvirus 6)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antibodies, Monoclonal/immunology
MH  - Antigens, Viral/*immunology
MH  - Base Sequence
MH  - Cerebrospinal Fluid/virology
MH  - Child, Preschool
MH  - DNA-Binding Proteins/analysis/genetics/*immunology
MH  - Epitope Mapping
MH  - Epitopes/immunology
MH  - Herpesviridae Infections/*virology
MH  - Herpesvirus 6, Human/classification/*immunology
MH  - Humans
MH  - Infant
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Peptides/chemical synthesis/chemistry/genetics
MH  - Sequence Analysis, DNA
MH  - Viral Proteins/analysis/genetics/*immunology
PMC - PMC87953
EDAT- 2001/04/03 10:00
MHDA- 2001/08/29 10:01
PMCR- 2001/04/01
CRDT- 2001/04/03 10:00
PHST- 2001/04/03 10:00 [pubmed]
PHST- 2001/08/29 10:01 [medline]
PHST- 2001/04/03 10:00 [entrez]
PHST- 2001/04/01 00:00 [pmc-release]
AID - 1558 [pii]
AID - 10.1128/JCM.39.4.1449-1455.2001 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2001 Apr;39(4):1449-55. doi: 10.1128/JCM.39.4.1449-1455.2001.