PMID- 11283269
OWN - NLM
STAT- MEDLINE
DCOM- 20010419
LR  - 20220225
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 21
IP  - 8
DP  - 2001 Apr
TI  - RBP1 recruits the mSIN3-histone deacetylase complex to the pocket of 
      retinoblastoma tumor suppressor family proteins found in limited discrete regions 
      of the nucleus at growth arrest.
PG  - 2918-32
AB  - Retinoblastoma (RB) tumor suppressor family pocket proteins induce cell cycle 
      arrest by repressing transcription of E2F-regulated genes through both histone 
      deacetylase (HDAC)-dependent and -independent mechanisms. In this study we have 
      identified a stable complex that accounts for the recruitment of both repression 
      activities to the pocket. One component of this complex is RBP1, a known 
      pocket-binding protein that exhibits both HDAC-dependent and -independent 
      repression functions. RB family proteins were shown to associate via the pocket 
      with previously identified mSIN3-SAP30-HDAC complexes containing exclusively 
      class I HDACs. Such enzymes do not interact directly with RB family proteins but 
      rather utilize RBP1 to target the pocket. This mechanism was shown to account for 
      the majority of RB-associated HDAC activity. We also show that in quiescent 
      normal human cells this entire RBP1-mSIN3-SAP30-HDAC complex colocalizes with 
      both RB family members and E2F4 in a limited number of discrete regions of the 
      nucleus that in other studies have been shown to represent the initial origins of 
      DNA replication following growth stimulation. These results suggest that RB 
      family members, at least in part, drive exit from the cell cycle by recruitment 
      of this HDAC complex via RBP1 to repress transcription from E2F-dependent 
      promoters and possibly to alter chromatin structure at DNA origins.
FAU - Lai, A
AU  - Lai A
AD  - Department of Biochemistry, McGill University, Montreal, Quebec, Canada H3G 1Y6.
FAU - Kennedy, B K
AU  - Kennedy BK
FAU - Barbie, D A
AU  - Barbie DA
FAU - Bertos, N R
AU  - Bertos NR
FAU - Yang, X J
AU  - Yang XJ
FAU - Theberge, M C
AU  - Theberge MC
FAU - Tsai, S C
AU  - Tsai SC
FAU - Seto, E
AU  - Seto E
FAU - Zhang, Y
AU  - Zhang Y
FAU - Kuzmichev, A
AU  - Kuzmichev A
FAU - Lane, W S
AU  - Lane WS
FAU - Reinberg, D
AU  - Reinberg D
FAU - Harlow, E
AU  - Harlow E
FAU - Branton, P E
AU  - Branton PE
LA  - eng
GR  - GM485180/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Carrier Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (E2F Transcription Factors)
RN  - 0 (E2F4 Transcription Factor)
RN  - 0 (E2F4 protein, human)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Retinoblastoma Protein)
RN  - 0 (Retinoblastoma-Binding Protein 1)
RN  - 0 (SAP30 protein, human)
RN  - 0 (Transcription Factor DP1)
RN  - 0 (Transcription Factors)
RN  - EC 3.5.1.98 (Histone Deacetylases)
RN  - EC 3.5.1.98 (Mi-2 Nucleosome Remodeling and Deacetylase Complex)
RN  - EC 3.5.1.98 (Sin3 Histone Deacetylase and Corepressor Complex)
SB  - IM
MH  - Binding Sites
MH  - Biological Transport, Active
MH  - *Carrier Proteins
MH  - *Cell Cycle Proteins
MH  - Cell Line
MH  - Cell Nucleus/metabolism
MH  - *DNA-Binding Proteins
MH  - E2F Transcription Factors
MH  - E2F4 Transcription Factor
MH  - Histone Deacetylases/chemistry/genetics/*metabolism
MH  - Humans
MH  - In Vitro Techniques
MH  - Interphase/*physiology
MH  - Macromolecular Substances
MH  - Mi-2 Nucleosome Remodeling and Deacetylase Complex
MH  - Models, Biological
MH  - Recombinant Fusion Proteins/chemistry/genetics/metabolism
MH  - Retinoblastoma Protein/*metabolism
MH  - Retinoblastoma-Binding Protein 1
MH  - Sin3 Histone Deacetylase and Corepressor Complex
MH  - Transcription Factor DP1
MH  - Transcription Factors/chemistry/genetics/*metabolism
PMC - PMC86920
EDAT- 2001/04/03 10:00
MHDA- 2001/04/21 10:01
PMCR- 2001/04/01
CRDT- 2001/04/03 10:00
PHST- 2001/04/03 10:00 [pubmed]
PHST- 2001/04/21 10:01 [medline]
PHST- 2001/04/03 10:00 [entrez]
PHST- 2001/04/01 00:00 [pmc-release]
AID - 1477 [pii]
AID - 10.1128/MCB.21.8.2918-2932.2001 [doi]
PST - ppublish
SO  - Mol Cell Biol. 2001 Apr;21(8):2918-32. doi: 10.1128/MCB.21.8.2918-2932.2001.