PMID- 11284470
OWN - NLM
STAT- MEDLINE
DCOM- 20010614
LR  - 20170214
IS  - 0148-6071 (Print)
IS  - 0148-6071 (Linking)
VI  - 25
IP  - 2
DP  - 2001 Mar-Apr
TI  - Total parenteral nutrition decreases liver oxidative metabolism and antioxidant 
      defenses in healthy rats: comparative effect of dietary olive and soybean oil.
PG  - 52-9
AB  - BACKGROUND: Total parenteral nutrition (TPN) is used for critically ill patients 
      undergoing surgery, after trauma, or during disease conditions that favor 
      oxidative stress. We studied the effect of TPN on liver oxidative metabolism and 
      antioxidant defenses in rats, and we compared the effect of soybean oil- and 
      olive oil-based diets. METHODS: Seven-week-old rats (n = 28) were divided into 
      four groups. Two experimental groups received a TPN solution containing soybean 
      oil (TPN-S) or a mixture of olive/soybean oil, 80/20 (TPN-O), IV for 6 days. 
      Orally fed animals received a solid diet including soybean oil (Oral-S) or 
      olive/soybean oil, 80/20 (Oral-O). The following parameters were measured: 
      DL-alpha-tocopherol, vitamin A, malondialdehyde and thiobarbituric acid reactive 
      substances (MDA-TBARS), and total radical-trapping antioxidant parameter (TRAP) 
      in serum; DL-alpha-tocopherol, vitamin A, glutathione (GSH), and catalase (Cat) 
      activity in liver homogenate; fatty acids from phospholipid, cytochrome P-450 
      content, NADPH-cytochrome c2 reductase activity in liver microsomes; superoxide 
      dismutase (SOD), glutathione peroxidase (Gpx), glutathione reductase (GR), 
      glutathione transferase (GST), and glucose-6-phosphate dehydrogenase (G6PD) in 
      liver cytosol. RESULTS: The soybean or olive oil diets modified the liver 
      microsomal fatty acid phospholipid composition, but the unsaturation index 
      remained unchanged. TPN specifically increased the saturation of the membrane. 
      The cytochrome P-450 level and the NADPH-cytochrome c2 reductase, SOD, Gpx, Cat, 
      and GST activities were unchanged by soybean oil or olive oil diet but decreased 
      receiving TPN. CONCLUSIONS: In rats, TPN decreased the liver oxidative metabolism 
      and enzymatic antioxidant defenses. This may be related to saturation of the 
      liver microsomal fatty acids.
FAU - Lespine, A
AU  - Lespine A
AD  - Laboratoire de Biochimie Generale et Nutritionnelle, CHU Purpan, Toulouse. 
      anne.lespine@wanadoo.fr
FAU - Fernandez, Y
AU  - Fernandez Y
FAU - Periquet, B
AU  - Periquet B
FAU - Galinier, A
AU  - Galinier A
FAU - Garcia, J
AU  - Garcia J
FAU - Anglade, F
AU  - Anglade F
FAU - Ghisolfi, J
AU  - Ghisolfi J
FAU - Thouvenot, J P
AU  - Thouvenot JP
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - JPEN J Parenter Enteral Nutr
JT  - JPEN. Journal of parenteral and enteral nutrition
JID - 7804134
RN  - 0 (Antioxidants)
RN  - 0 (Lipids)
RN  - 0 (Olive Oil)
RN  - 0 (Plant Oils)
RN  - 8001-22-7 (Soybean Oil)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Antioxidants/*metabolism
MH  - Lipids/chemistry
MH  - Liver/*metabolism
MH  - Male
MH  - Olive Oil
MH  - Oxidation-Reduction
MH  - Oxidative Stress
MH  - *Parenteral Nutrition, Total
MH  - Plant Oils/*administration & dosage
MH  - Rats
MH  - Rats, Wistar
MH  - Soybean Oil/*administration & dosage
EDAT- 2001/04/04 10:00
MHDA- 2001/06/23 10:01
CRDT- 2001/04/04 10:00
PHST- 2001/04/04 10:00 [pubmed]
PHST- 2001/06/23 10:01 [medline]
PHST- 2001/04/04 10:00 [entrez]
AID - 10.1177/014860710102500252 [doi]
PST - ppublish
SO  - JPEN J Parenter Enteral Nutr. 2001 Mar-Apr;25(2):52-9. doi: 
      10.1177/014860710102500252.