PMID- 11284916
OWN - NLM
STAT- MEDLINE
DCOM- 20010419
LR  - 20200315
IS  - 0960-7722 (Print)
IS  - 1365-2184 (Electronic)
IS  - 0960-7722 (Linking)
VI  - 34
IP  - 1
DP  - 2001 Feb
TI  - SERCA activity is required for timely progression through G1/S.
PG  - 15-30
AB  - Changes in intracellular Ca2+ correlate with specific events in the cell cycle. 
      Here we investigated the role of Ca2+ in the G1 phase. HEK 293 cells were 
      arrested in mitosis and subjected to short-term treatments that alter Ca2+ 
      homeostasis prior to their release into G1. Treatment with thapsigargin (TG), an 
      irreversible inhibitor of the sarco-endoplasmic reticulum Ca2+ ATPase (SERCA) 
      lengthened the G1 phase. Moreover, TG treatment also resulted in a dramatic 
      alteration in cellular morphology and attachment and in the reduction of MAPK 
      activity and lower levels of cyclin D1 and cyclin E proteins. Treatments with 
      reagents that transiently increase or decrease cytosolic Ca2+ or that temporarily 
      inactivate SERCA did not alter any of the above parameters. Cells expressing a 
      TG-resistant form of SERCA progressed normally through the G1/S transition after 
      TG treatment. These results suggest that long-term SERCA inactivation affects 
      cell cycle-dependent events and compromises progression through G1/S.
FAU - Simon, V R
AU  - Simon VR
AD  - Banting and Best Department of Medical Research, University of Toronto, ON, 
      Canada. simon_viviana@hotmail.com
FAU - Moran, M F
AU  - Moran MF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cell Prolif
JT  - Cell proliferation
JID - 9105195
RN  - 0 (Cyclin E)
RN  - 136601-57-5 (Cyclin D1)
RN  - 67526-95-8 (Thapsigargin)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 3.6.3.8 (Sarcoplasmic Reticulum Calcium-Transporting ATPases)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Calcium/metabolism
MH  - Calcium-Transporting ATPases/*metabolism
MH  - Cell Line
MH  - Cyclin D1/metabolism
MH  - Cyclin E/metabolism
MH  - *G1 Phase/drug effects
MH  - Humans
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - *S Phase/drug effects
MH  - Sarcoplasmic Reticulum Calcium-Transporting ATPases
MH  - Thapsigargin/pharmacology
PMC - PMC6495481
EDAT- 2001/04/04 10:00
MHDA- 2001/04/21 10:01
PMCR- 2001/12/20
CRDT- 2001/04/04 10:00
PHST- 2001/04/04 10:00 [pubmed]
PHST- 2001/04/21 10:01 [medline]
PHST- 2001/04/04 10:00 [entrez]
PHST- 2001/12/20 00:00 [pmc-release]
AID - cpr192 [pii]
AID - CPR192 [pii]
AID - 10.1046/j.1365-2184.2001.00192.x [doi]
PST - ppublish
SO  - Cell Prolif. 2001 Feb;34(1):15-30. doi: 10.1046/j.1365-2184.2001.00192.x.