PMID- 11286567
OWN - NLM
STAT- MEDLINE
DCOM- 20010705
LR  - 20041117
IS  - 0143-4004 (Print)
IS  - 0143-4004 (Linking)
VI  - 22
IP  - 4
DP  - 2001 Apr
TI  - Differential expression of lactate dehydrogenase isozymes (LDH) in human placenta 
      with high expression of LDH-A(4) isozyme in the endothelial cells of 
      pre-eclampsia villi.
PG  - 317-22
AB  - To evaluate the role of LDH isozymes in the human placenta during the third 
      trimester, placentae were obtained from patients with normal pregnancy and 
      pre-eclampsia. LDH-A(4)isozyme was immunolocalized primarily in the fetal 
      endothelial cells while LDH-B(4)isozyme was predominantly present in 
      syncytiotrophoblasts. This distinct cellular expression pattern of LDH isozymes 
      was confirmed in HUVE and JEG cells. In addition to demonstrating the presence of 
      five LDH isozymes in the placenta, zymograms showed that there was predominant 
      activity of LDH-A(4)isozyme in HUVE cells and high activity of LDH-B(4)in JEG 
      cells. Quantitative studies of LDH by agarose gel electrophoresis and Northern 
      analysis in patients concluded that LDH-A(4)isozyme was increased in 
      pre-eclampsia. The LDH-A(4)isozyme activity increased (P< 0.01) approx 1.6-fold 
      in pre-eclampsia but there was no difference in the LDH-B(4)isozyme activity 
      between placentae from normal compared to pre-eclampsia pregnancy. The level of 
      LDH-A mRNA was increased (P< 0.05) approx twofold in pre-eclampsia. We conclude 
      that the LDH-A gene in the endothelial cells of the placenta within the fetal 
      microvasculature is increased in pre-eclampsia, probably as a result of hypoxia. 
      LDH-A(4)isozyme activity and gene expression in placental endothelial cells, 
      therefore, is a marker for the endothelial pathology seen in pre-eclampsia.
CI  - Copyright 2001 Harcourt Publishers Ltd.
FAU - Tsoi, S C
AU  - Tsoi SC
AD  - Perinatal Research Laboratories, Department of Obstetrics and Gynecology, 
      University of Wisconsin-Madison, Madison, WI 53715, USA.
FAU - Zheng, J
AU  - Zheng J
FAU - Xu, F
AU  - Xu F
FAU - Kay, H H
AU  - Kay HH
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Placenta
JT  - Placenta
JID - 8006349
RN  - 0 (Isoenzymes)
RN  - 0 (RNA, Messenger)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
SB  - IM
MH  - Blotting, Northern
MH  - Chorionic Villi/*enzymology
MH  - Electrophoresis, Agar Gel
MH  - Endothelium, Vascular/*enzymology
MH  - Female
MH  - *Gene Expression
MH  - Humans
MH  - Immunohistochemistry
MH  - Isoenzymes/*genetics/metabolism
MH  - L-Lactate Dehydrogenase/*genetics/metabolism
MH  - Placenta/*enzymology
MH  - Polymerase Chain Reaction
MH  - Pre-Eclampsia/*enzymology
MH  - Pregnancy
MH  - RNA, Messenger/analysis
EDAT- 2001/04/05 10:00
MHDA- 2001/07/06 10:01
CRDT- 2001/04/05 10:00
PHST- 2001/04/05 10:00 [pubmed]
PHST- 2001/07/06 10:01 [medline]
PHST- 2001/04/05 10:00 [entrez]
AID - S0143-4004(00)90620-9 [pii]
AID - 10.1053/plac.2000.0620 [doi]
PST - ppublish
SO  - Placenta. 2001 Apr;22(4):317-22. doi: 10.1053/plac.2000.0620.