PMID- 11287309
OWN - NLM
STAT- MEDLINE
DCOM- 20010531
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 491
IP  - 1-2
DP  - 2001 Apr 5
TI  - Genotoxic effects of styrene-7,8-oxide in human white blood cells: comet assay in 
      relation to the induction of sister-chromatid exchanges and micronuclei.
PG  - 163-72
AB  - Styrene is used in the production of plastics, resins and rubber. The highest 
      human exposures to styrene take place by inhalation during the production of 
      fiberglass reinforced plastics. Styrene is metabolized mainly in the liver to 
      styrene-7,8-oxide (SO), its principal in vivo mutagenic metabolite. In this 
      study, human peripheral white blood cells were exposed to several SO 
      concentrations (10-200 microM) in order to evaluate its genotoxic properties by 
      means of comet assay, sister-chromatid exchanges (SCE) and cytokinesis-blocked 
      micronucleus (MN) test, in addition to determine its clastogenic or aneugenic 
      properties by combining MN with fluorescence in situ hybridization (FISH) 
      procedures. Our results show that SO induces DNA damage, SCE and MN in human 
      leukocytes in vitro at concentrations above 50 microM, and that there is a strong 
      relationship between DNA damage, as measured by the comet assay, and cytogenetic 
      damage induced by SO at the doses employed. SO shows preferentially a clastogenic 
      activity and produces a cytostatic effect at high doses, reflected by the 
      significant decrease of the calculated proliferation indices. A good dose-effect 
      relationship is obtained in the three tests performed at the concentration range 
      assayed.
FAU - Laffon, B
AU  - Laffon B
AD  - Dpto. Biologia Celular y Molecular, Facultad de Ciencias, Universidade da Coruna, 
      Campus da Zapateira s/n, 15071 A, Coruna, Spain.
FAU - Pasaro, E
AU  - Pasaro E
FAU - Mendez, J
AU  - Mendez J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Epoxy Compounds)
RN  - 0 (Mutagens)
RN  - 9QH06NGT6O (styrene oxide)
SB  - IM
MH  - Adult
MH  - Comet Assay
MH  - Epoxy Compounds/*toxicity
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukocytes/*drug effects/ultrastructure
MH  - Male
MH  - *Micronuclei, Chromosome-Defective
MH  - Mutagens/*toxicity
MH  - *Sister Chromatid Exchange
EDAT- 2001/04/05 10:00
MHDA- 2001/06/02 10:01
CRDT- 2001/04/05 10:00
PHST- 2001/04/05 10:00 [pubmed]
PHST- 2001/06/02 10:01 [medline]
PHST- 2001/04/05 10:00 [entrez]
AID - S1383571801001358 [pii]
AID - 10.1016/s1383-5718(01)00135-8 [doi]
PST - ppublish
SO  - Mutat Res. 2001 Apr 5;491(1-2):163-72. doi: 10.1016/s1383-5718(01)00135-8.