PMID- 11288128
OWN - NLM
STAT- MEDLINE
DCOM- 20010705
LR  - 20041117
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 21
IP  - 4
DP  - 2001 Apr
TI  - Role of amniotic fluid interphase fluorescence in situ hybridization (FISH) 
      analysis in patient management.
PG  - 327-32
AB  - We retrospectively reviewed 309 amniotic fluid interphase fluorescence in situ 
      hybridization (FISH) analyses performed from October 1995 to June 1999 to assess 
      the role of interphase FISH in the management of patients at increased risk for 
      fetal aneuploidies. Gestational age and indications for amniocentesis, clinical 
      interventions after FISH results, as well as interventions after final culture 
      reports were analyzed. There were 244 (79%) normal, 50 (16%) abnormal and 15 (5%) 
      inconclusive FISH results. There were no false-positive or false-negative 
      results, but there were nine (3%) clinically significant chromosomal 
      abnormalities not detectable by FISH. Of the 50 women with abnormal FISH results, 
      26 (52%) elected to terminate the pregnancy prior to the availability of the 
      standard chromosome analysis. In two of the fetuses with trisomy 21 no 
      abnormalities were reported by ultrasound examination. Our experience indicates 
      that interphase FISH results played an important role in decision making, 
      especially for pregnancies close to 24 weeks' gestation. Standard karyotype 
      analysis is still required for detection of chromosome abnormalities not 
      detectable by interphase FISH techniques and for clarification of unusual or 
      inconclusive FISH results.
CI  - Copyright 2001 John Wiley & Sons, Ltd.
FAU - Cheong Leung, W
AU  - Cheong Leung W
AD  - Fetal Assessment & Treatment Unit, Department of Obstetrics & Gynaecology, Mount 
      Sinai Hospital, University of Toronto, Toronto, Canada.
FAU - Chitayat, D
AU  - Chitayat D
FAU - Seaward, G
AU  - Seaward G
FAU - Windrim, R
AU  - Windrim R
FAU - Ryan, G
AU  - Ryan G
FAU - Barrett, J
AU  - Barrett J
FAU - Winsor, E J
AU  - Winsor EJ
LA  - eng
PT  - Journal Article
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
SB  - IM
MH  - Amniotic Fluid/*cytology
MH  - *Aneuploidy
MH  - *Chromosome Aberrations
MH  - Female
MH  - Gestational Age
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - *Interphase
MH  - Karyotyping
MH  - Pregnancy
MH  - Retrospective Studies
MH  - Ultrasonography, Prenatal
EDAT- 2001/04/05 10:00
MHDA- 2001/07/06 10:01
CRDT- 2001/04/05 10:00
PHST- 2001/04/05 10:00 [pubmed]
PHST- 2001/07/06 10:01 [medline]
PHST- 2001/04/05 10:00 [entrez]
AID - 10.1002/pd.58 [doi]
PST - ppublish
SO  - Prenat Diagn. 2001 Apr;21(4):327-32. doi: 10.1002/pd.58.