PMID- 11292531
OWN - NLM
STAT- MEDLINE
DCOM- 20010628
LR  - 20190831
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 78
IP  - 3-4
DP  - 2001 Feb 10
TI  - Cytokine mRNA levels in isolated feline monocytes.
PG  - 305-15
AB  - Real-time PCR systems were developed to quantitate cytokine expression in 
      short-time cultivated feline monocytes. Feline-specific interleukin-1beta 
      (IL-1beta), IL-6, and tumor necrosis factor-alpha (TNF-alpha) primers as well as 
      TaqMan probes were designed and were adapted to a quantitative PCR system which 
      had been previously established for feline IL-10 and IL-12 p40. Quantitative 
      analysis of cytokine messenger RNA (mRNA) transcription based on the comparison 
      of the cytokine with the housekeeping gene feline glyceraldehyde-3-phosphate 
      dehydrogenase (GAPDH), providing universally expressed mRNA. GAPDH mRNA was 
      readily detectable in cDNA prepared from short-time cultivated peripheral blood 
      monocytes. Cytokine mRNA was demonstrated in all samples at variable amounts. 
      IL-1beta and TNF-alpha mRNA was constitutively expressed whereas IL-6, IL-10 and 
      IL-12 p40 mRNA was generally expressed at a lower level and was occasionally not 
      detected. There was a great variability of cytokine production between individual 
      cats and at different time points in the same cat.
FAU - Kipar, A
AU  - Kipar A
AD  - Institute for Veterinary Pathology, Frankfurter Strasse 96, Justus-Liebig 
      University Giessen, D-35392 Giessen, Germany. anja.kipar@vetmed.uni-giessen.de
FAU - Leutenegger, C M
AU  - Leutenegger CM
FAU - Hetzel, U
AU  - Hetzel U
FAU - Akens, M K
AU  - Akens MK
FAU - Mislin, C N
AU  - Mislin CN
FAU - Reinacher, M
AU  - Reinacher M
FAU - Lutz, H
AU  - Lutz H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (RNA primers)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 63231-63-0 (RNA)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
SB  - IM
MH  - Animals
MH  - Cats/*blood
MH  - Cell Separation/veterinary
MH  - Cytokines/*genetics
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/genetics
MH  - Interleukin-1/genetics
MH  - Interleukin-6/genetics
MH  - Monocytes/*metabolism
MH  - Polymerase Chain Reaction/veterinary
MH  - RNA
MH  - RNA, Messenger/*metabolism
MH  - Tumor Necrosis Factor-alpha/genetics
EDAT- 2001/04/09 10:00
MHDA- 2001/06/29 10:01
CRDT- 2001/04/09 10:00
PHST- 2001/04/09 10:00 [pubmed]
PHST- 2001/06/29 10:01 [medline]
PHST- 2001/04/09 10:00 [entrez]
AID - S0165242701002409 [pii]
AID - 10.1016/s0165-2427(01)00240-9 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2001 Feb 10;78(3-4):305-15. doi: 
      10.1016/s0165-2427(01)00240-9.