PMID- 11292535
OWN - NLM
STAT- MEDLINE
DCOM- 20010628
LR  - 20191210
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 78
IP  - 3-4
DP  - 2001 Feb 10
TI  - Measurement of canine IgE using the alpha chain of the human high affinity IgE 
      receptor.
PG  - 349-55
AB  - In vitro assays for allergen specific immunoglobulin E (IgE) are a convenient and 
      reproducible alternative to intradermal skin testing in dogs. Such tests may be 
      used to support a diagnosis of atopic dermatitis and to define appropriate 
      allergens for immunotherapy. Current in vitro assays rely upon monoclonal or 
      polyclonal antibodies as IgE detection reagents. However, in sera where 
      allergen-specific IgG occurs in great excess, any IgE:IgG cross-reactivity of the 
      detection reagent may result in lowered assay specificity. Therefore, we have 
      developed an assay for canine IgE which uses a recombinant form of the 
      extracellular part of the alpha chain of the human high affinity IgE receptor 
      (FcvarepsilonRIalpha). Biotinylated FcvarepsilonRIalpha shows no significant 
      binding to purified canine IgG, and recognizes a heat labile antibody in serum, 
      with a detection limit of 73-146pg/ml. Comparison of assay signals using the 
      labeled FcvarepsilonRIalpha and a highly specific anti-canine IgE monoclonal 
      antibody (MAb) shows good agreement. The FcvarepsilonRIalpha is therefore a 
      sensitive and specific alternative to polyclonal or monoclonal antibodies for 
      canine serum IgE measurement.
FAU - Stedman, K
AU  - Stedman K
AD  - Heska Corporation, 1613 Prospect Parkway, Fort Collins, CO 80525, USA. 
      mccallc@heska.com
FAU - Lee, K
AU  - Lee K
FAU - Hunter, S
AU  - Hunter S
FAU - Rivoire, B
AU  - Rivoire B
FAU - McCall, C
AU  - McCall C
FAU - Wassom, D
AU  - Wassom D
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Immunoglobulin alpha-Chains)
RN  - 0 (Receptors, IgE)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Dogs/*immunology
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Humans
MH  - Immunoglobulin E/*analysis/blood
MH  - Immunoglobulin alpha-Chains/*analysis
MH  - Protein Conformation
MH  - Receptors, IgE/*chemistry
EDAT- 2001/04/09 10:00
MHDA- 2001/06/29 10:01
CRDT- 2001/04/09 10:00
PHST- 2001/04/09 10:00 [pubmed]
PHST- 2001/06/29 10:01 [medline]
PHST- 2001/04/09 10:00 [entrez]
AID - S0165242701002422 [pii]
AID - 10.1016/s0165-2427(01)00242-2 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2001 Feb 10;78(3-4):349-55. doi: 
      10.1016/s0165-2427(01)00242-2.