PMID- 11294474
OWN - NLM
STAT- MEDLINE
DCOM- 20010816
LR  - 20220410
IS  - 0963-6897 (Print)
IS  - 0963-6897 (Linking)
VI  - 10
IP  - 1
DP  - 2001 Jan-Feb
TI  - Engraftment and albumin production of intrasplenically transplanted rat 
      hepatocytes (Sprague-Dawley), freshly isolated versus cryopreserved, into Nagase 
      analbuminemic rats (NAR).
PG  - 67-80
AB  - Banking of cryopreserved hepatocytes is a prerequisite for large-scale hepatocyte 
      transplantation in the clinic. We compared the efficacy of intrasplenic 
      transplantation into Nagase analbuminemic rats (NAR) of freshly isolated (FIH) 
      and cryopreserved (CH) hepatocytes. Hepatocytes were cryopreserved using a 
      controlled rate freezing protocol. Albumin production of thawed CH and FIH was 
      measured in vitro in culture by ELISA and by Western blot. After in vivo 
      intrasplenic transplantation of NAR with either FIH or CH we assessed 1) albumin 
      in the serum of recipients by ELISA and by Western blotting analysis at different 
      time intervals, and 2) hepatocyte engraftment by albumin immunohistochemical 
      staining into spleens and livers at euthanasia. In vitro, albumin was produced up 
      to day 4 of culture in both CH and FIH. In vivo, no intrasplenic engraftment of 
      hepatocytes occurred. Intrahepatic engraftment of CH (cell number/mm2) was 
      significantly (twofold) lower than that of FIH and appeared only as isolated 
      cells and small (<10 cells) clusters, while bigger clusters (>10 cells) were 
      observed with FIH. In the FIH group, serum albumin production was observed up to 
      32-49 days posttransplantation while in the CH group no serum albumin production 
      was detected. Our results emphasize the need to improve 1) hepatocyte 
      transplantation procedures either by repeated hepatocytes injections and/or by 
      transplantation under a regeneration response, and 2) the freeze/thaw protocols 
      of hepatocytes.
FAU - David, P
AU  - David P
AD  - Laboratoire de Chirurgie Experimentale, Fondation Transplantation, Strasbourg, 
      France.
FAU - Alexandre, E
AU  - Alexandre E
FAU - Audet, M
AU  - Audet M
FAU - Chenard-Neu, M P
AU  - Chenard-Neu MP
FAU - Wolf, P
AU  - Wolf P
FAU - Jaeck, D
AU  - Jaeck D
FAU - Azimzadeh, A
AU  - Azimzadeh A
FAU - Richert, L
AU  - Richert L
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
RN  - 0 (Serum Albumin)
SB  - IM
MH  - Animals
MH  - Cell Separation
MH  - Cell Transplantation/*methods
MH  - Cells, Cultured
MH  - Cryopreservation
MH  - Graft Survival
MH  - Hepatocytes/pathology/*transplantation
MH  - Humans
MH  - In Vitro Techniques
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Serum Albumin/biosynthesis/*deficiency
MH  - Spleen/pathology/surgery
EDAT- 2001/04/11 10:00
MHDA- 2001/08/17 10:01
CRDT- 2001/04/11 10:00
PHST- 2001/04/11 10:00 [pubmed]
PHST- 2001/08/17 10:01 [medline]
PHST- 2001/04/11 10:00 [entrez]
PST - ppublish
SO  - Cell Transplant. 2001 Jan-Feb;10(1):67-80.