PMID- 11295195
OWN - NLM
STAT- MEDLINE
DCOM- 20010607
LR  - 20191104
IS  - 0167-7012 (Print)
IS  - 0167-7012 (Linking)
VI  - 45
IP  - 1
DP  - 2001 May
TI  - Rapid detection, identification, and enumeration of Escherichia coli by 
      fluorescence in situ hybridization using an array scanner.
PG  - 31-9
AB  - A new fluorescence in situ hybridization (FISH) method using peptide nucleic acid 
      (PNA) probes and an array scanner for rapid detection, identification, and 
      enumeration of Escherichia coli is described. The test utilizes Cy3-labeled 
      peptide nucleic acid (PNA) probes complementary to a specific 16S rRNA sequence 
      of E. coli. Samples were filtered and incubated for 5 h, the membrane filters 
      were then analyzed by fluorescence in situ hybridization and results were 
      visualized with an array scanner. Results were provided as fluorescent spots 
      representing E. coli microcolonies on the membrane filter surface. The number of 
      fluorescent spots correlated to standard colony counts up to 100 colony-forming 
      units per membrane filter. Above this level, better accuracy was obtained with 
      PNA FISH due to the ability of the scanner to resolve neighboring microcolonies, 
      which were not distinguishable as individual colonies once they were visible by 
      eye.
FAU - Stender, H
AU  - Stender H
AD  - Boston Probes Inc., 75E Wiggins Avenue, Bedford, MA 01730, USA. 
      HStender@BostonProbes.com
FAU - Oliveira, K
AU  - Oliveira K
FAU - Rigby, S
AU  - Rigby S
FAU - Bargoot, F
AU  - Bargoot F
FAU - Coull, J
AU  - Coull J
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Microbiol Methods
JT  - Journal of microbiological methods
JID - 8306883
RN  - 0 (Peptide Nucleic Acids)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Colony Count, Microbial/methods
MH  - Escherichia coli/genetics/growth & development/*isolation & purification
MH  - Image Processing, Computer-Assisted
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Microscopy, Fluorescence
MH  - Peptide Nucleic Acids/*chemistry
MH  - RNA, Ribosomal, 16S/chemistry/genetics
EDAT- 2001/04/11 10:00
MHDA- 2001/06/19 10:01
CRDT- 2001/04/11 10:00
PHST- 2001/04/11 10:00 [pubmed]
PHST- 2001/06/19 10:01 [medline]
PHST- 2001/04/11 10:00 [entrez]
AID - S0167-7012(01)00218-4 [pii]
AID - 10.1016/s0167-7012(01)00218-4 [doi]
PST - ppublish
SO  - J Microbiol Methods. 2001 May;45(1):31-9. doi: 10.1016/s0167-7012(01)00218-4.