PMID- 11295236
OWN - NLM
STAT- MEDLINE
DCOM- 20010913
LR  - 20190826
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 88
IP  - 1-2
DP  - 2001 Mar 31
TI  - Dose-dependent induction of mRNAs encoding brain-derived neurotrophic factor and 
      heat-shock protein-72 after cortical spreading depression in the rat.
PG  - 103-12
AB  - Previous studies have demonstrated that cortical spreading depression (CSD) 
      increases the expression of putative neuroprotective proteins. The objective of 
      the present study was to elucidate the relationship between the number of 
      episodes of CSD and steady-state levels of mRNAs encoding brain-derived 
      neurotrophic factor (BDNF), heat-shock protein-72 (hsp72) and c-fos. Wistar rats 
      were administered one, five, or twenty-five episodes of CSD evoked by application 
      of 2 M KCl to the frontal cortex of one hemisphere. Animals were permitted to 
      recover for 30 min, 2 h or 24 h prior to sacrifice. Total RNA was isolated from 
      the parietal cortex of each hemisphere and analyzed using Northern blots. At 30 
      min recovery, levels of BDNF mRNA were not significantly elevated after 1 episode 
      of CSD, but were increased 4-fold after five episodes of CSD and 11-fold after 
      twenty-five episodes of CSD, relative to levels in the contralateral hemisphere. 
      At 2 h recovery, BDNF mRNA levels increased 2-, 3- and 9-fold, respectively. At 
      24 h, BDNF mRNA had returned to control levels in all groups. Thus, CSD increased 
      levels of BDNF mRNA in a dose-dependent fashion at the early recovery times. 
      Hsp72 mRNA was below the level of detection after 1 and 5 episodes of CSD. 
      However, after twenty-five episodes of CSD, hsp72 mRNA levels were increased in 
      the ipsilateral hemisphere at 30 min and 2 h recovery. Unlike levels of BDNF and 
      hsp72 mRNA, levels of c-fos mRNA were increased nearly to the same extent at 30 
      min and 2 h after one, five or twenty-five episodes of CSD before returning to 
      control by 24 h recovery. These results demonstrate that CSD triggers a 
      dose-dependent increase in the expression of genes encoding neuroprotective 
      proteins, which may mediate tolerance to ischemia induced by CSD.
FAU - Rangel, Y M
AU  - Rangel YM
AD  - Department of Neurosurgery, University of Pennsylvania School of Medicine, 
      Philadelphia, PA 19104-6070, USA.
FAU - Kariko, K
AU  - Kariko K
FAU - Harris, V A
AU  - Harris VA
FAU - Duvall, M E
AU  - Duvall ME
FAU - Welsh, F A
AU  - Welsh FA
LA  - eng
GR  - EY06644/EY/NEI NIH HHS/United States
GR  - NS08803/NS/NINDS NIH HHS/United States
GR  - NS29331/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (HSP72 Heat-Shock Proteins)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Brain Chemistry/genetics
MH  - Brain Ischemia/physiopathology
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - Cerebrovascular Circulation/physiology
MH  - Cortical Spreading Depression/*physiology
MH  - HSP72 Heat-Shock Proteins
MH  - Heat-Shock Proteins/*genetics
MH  - Laser-Doppler Flowmetry
MH  - Male
MH  - Proto-Oncogene Proteins c-fos/genetics
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Time Factors
EDAT- 2001/04/11 10:00
MHDA- 2001/09/14 10:01
CRDT- 2001/04/11 10:00
PHST- 2001/04/11 10:00 [pubmed]
PHST- 2001/09/14 10:01 [medline]
PHST- 2001/04/11 10:00 [entrez]
AID - S0169328X01000377 [pii]
AID - 10.1016/s0169-328x(01)00037-7 [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 2001 Mar 31;88(1-2):103-12. doi: 
      10.1016/s0169-328x(01)00037-7.