PMID- 11295250
OWN - NLM
STAT- MEDLINE
DCOM- 20010913
LR  - 20220330
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 88
IP  - 1-2
DP  - 2001 Mar 31
TI  - Cultured olfactory ensheathing cells express nerve growth factor, brain-derived 
      neurotrophic factor, glia cell line-derived neurotrophic factor and their 
      receptors.
PG  - 203-13
AB  - In the primary olfactory pathway axons of olfactory neurons (ONs) are accompanied 
      by ensheathing cells (ECs) as the fibres course towards the olfactory bulb. 
      Ensheathing cells are thought to play an important role in promoting and guiding 
      olfactory axons to their appropriate target. In recent years, studies have shown 
      that transplants of ECs into lesions in the central nervous system (CNS) are able 
      to stimulate the growth of axons and in some cases restore functional 
      connections. In an attempt to identify a possible mechanism underlying EC support 
      for olfactory nerve growth and CNS axonal regeneration, this study investigated 
      the production of growth factors and expression of corresponding receptors by 
      these cells. Three techniques immunohistochemistry, enzyme linked immunosorbent 
      assay (ELISA) and reverse transcriptase-polymerase chain reaction (RT-PCR) were 
      used to assess growth factor expression in cultured ECs. Immunohistochemistry 
      showed that ECs expressed nerve growth factor (NGF), brain derived neurotrophic 
      factor (BDNF) and glial cell-line derived neurotrophic factor (GDNF). ELISA 
      confirmed the intracellular presence of NGF and BDNF and showed that, compared to 
      BDNF, about seven times as much NGF was secreted by ECs. RT-PCR analysis 
      demonstrated expression of mRNA for NGF, BDNF, GDNF and neurturin (NTN). In 
      addition, ECs also expressed the receptors trkB, GFRalpha-1 and GFRalpha-2. The 
      results of the experiments show that ECs express a number of growth factors and 
      that BDNF in particular could act both in a paracrine and autocrine manner.
FAU - Woodhall, E
AU  - Woodhall E
AD  - Department of Anatomy and Physiology, University of Tasmania, P.O. Box 252-24, 
      Hobart, Tasmania 7001, Australia.
FAU - West, A K
AU  - West AK
FAU - Chuah, M I
AU  - Chuah MI
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Gdnf protein, rat)
RN  - 0 (Gfra1 protein, rat)
RN  - 0 (Gfra2 protein, rat)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor Receptors)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neurturin)
RN  - 0 (Nrtn protein, rat)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-ret)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptor, trkA)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.10.1 (Receptor, trkC)
RN  - EC 2.7.10.1 (Ret protein, Drosophila)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/analysis/genetics
MH  - Cells, Cultured
MH  - *Drosophila Proteins
MH  - Gene Expression/physiology
MH  - Glial Cell Line-Derived Neurotrophic Factor
MH  - Glial Cell Line-Derived Neurotrophic Factor Receptors
MH  - Nerve Growth Factor/analysis/genetics
MH  - Nerve Growth Factors/analysis/*genetics
MH  - Nerve Tissue Proteins/analysis/genetics
MH  - Neurturin
MH  - Olfactory Pathways/chemistry/*cytology/*physiology
MH  - Proto-Oncogene Proteins/analysis/genetics
MH  - Proto-Oncogene Proteins c-ret
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor Protein-Tyrosine Kinases/analysis/genetics
MH  - Receptor, trkA/analysis/genetics
MH  - Receptor, trkB/analysis/genetics
MH  - Receptor, trkC/analysis/genetics
MH  - Receptors, Nerve Growth Factor/analysis/*genetics
EDAT- 2001/04/11 10:00
MHDA- 2001/09/14 10:01
CRDT- 2001/04/11 10:00
PHST- 2001/04/11 10:00 [pubmed]
PHST- 2001/09/14 10:01 [medline]
PHST- 2001/04/11 10:00 [entrez]
AID - S0169328X01000444 [pii]
AID - 10.1016/s0169-328x(01)00044-4 [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 2001 Mar 31;88(1-2):203-13. doi: 
      10.1016/s0169-328x(01)00044-4.