PMID- 11295477
OWN - NLM
STAT- MEDLINE
DCOM- 20010524
LR  - 20190831
IS  - 0278-6915 (Print)
IS  - 0278-6915 (Linking)
VI  - 39
IP  - 4
DP  - 2001 Apr
TI  - Skin sensitisation testing--new perspectives and recommendations.
PG  - 293-301
AB  - Various methodological aspects of skin sensitisation testing have been explored, 
      particularly in the context of animal welfare considerations and reliability and 
      sensitivity of test methods. Recommendations are made for the conduct of current 
      and proposed OECD skin sensitisation tests with respect to appropriate test 
      configurations for the purposes of hazard identification and labelling, and the 
      requirement for positive controls. Specifically, the following aspects of guinea 
      pig sensitisation test methods have been addressed: (1) the number of test and 
      control animals required; (2) the option of using joint positive controls between 
      independent laboratories; (3) the choice of positive control chemicals; (4) the 
      optimal conduct and interpretation of rechallenge; and (5) the requirement for 
      pretreatment with sodium lauryl sulfate. In addition, the use of the murine local 
      lymph node assay (LLNA) has been considered. A number of conclusions have been 
      drawn and recommendations made as follows: In many instances, particularly with 
      the conduct of the guinea pig maximisation test, it is acceptable to halve the 
      number of test and control animals used. An optional scheme for the conduct of 
      joint positive control studies within a co-ordinated group of laboratories is 
      appropriate. Only one positive control chemical (alpha-hexyl cinnamic aldehyde) 
      is necessary for the routine assessment of assay sensitivity. The proper conduct 
      and interpretation of rechallenge can provide valuable information and 
      confirmation of results in guinea pig sensitisation tests. Sodium lauryl sulfate 
      should no longer be used as a pretreatment in the guinea pig maximisation test. 
      The LLNA is a viable and complete alternative to traditional guinea pig test 
      methods for the purposes of skin sensitisation hazard identification. These 
      recommendations provide the opportunity for both animal welfare benefits and 
      improved hazard identification.
FAU - Steiling, W
AU  - Steiling W
AD  - Henkel KGaA, Henkelstrasse 67, D-40191, Dusseldorf, Germany.
FAU - Basketter, D
AU  - Basketter D
FAU - Berthold, K
AU  - Berthold K
FAU - Butler, M
AU  - Butler M
FAU - Garrigue, J L
AU  - Garrigue JL
FAU - Kimber, I
AU  - Kimber I
FAU - Lea, L
AU  - Lea L
FAU - Newsome, C
AU  - Newsome C
FAU - Roggeband, R
AU  - Roggeband R
FAU - Stropp, G
AU  - Stropp G
FAU - Waterman, S
AU  - Waterman S
FAU - Wiemann, C
AU  - Wiemann C
LA  - eng
PT  - Guideline
PT  - Journal Article
PT  - Review
PL  - England
TA  - Food Chem Toxicol
JT  - Food and chemical toxicology : an international journal published for the British 
      Industrial Biological Research Association
JID - 8207483
RN  - 0 (Allergens)
RN  - 0 (Surface-Active Agents)
RN  - 368GB5141J (Sodium Dodecyl Sulfate)
SB  - IM
MH  - Allergens/*toxicity
MH  - Animal Welfare
MH  - Animals
MH  - Dermatitis, Allergic Contact/prevention & control
MH  - Disease Models, Animal
MH  - Guinea Pigs
MH  - Local Lymph Node Assay
MH  - Mice
MH  - Skin Tests/*methods/standards
MH  - Sodium Dodecyl Sulfate/*administration & dosage/toxicity
MH  - Surface-Active Agents/administration & dosage/toxicity
RF  - 57
EDAT- 2001/04/11 10:00
MHDA- 2001/05/26 10:01
CRDT- 2001/04/11 10:00
PHST- 2001/04/11 10:00 [pubmed]
PHST- 2001/05/26 10:01 [medline]
PHST- 2001/04/11 10:00 [entrez]
AID - S0278691500001472 [pii]
AID - 10.1016/s0278-6915(00)00147-2 [doi]
PST - ppublish
SO  - Food Chem Toxicol. 2001 Apr;39(4):293-301. doi: 10.1016/s0278-6915(00)00147-2.