PMID- 11296859
OWN - NLM
STAT- MEDLINE
DCOM- 20010628
LR  - 20181113
IS  - 0962-8452 (Print)
IS  - 1471-2954 (Electronic)
IS  - 0962-8452 (Linking)
VI  - 268
IP  - 1466
DP  - 2001 Mar 7
TI  - Association between major histocompatibility complex class IIB alleles and 
      resistance to Aeromonas salmonicida in Atlantic salmon.
PG  - 479-85
AB  - We have tested the importance of genetic variation in the major 
      histocompatibility complex (MHC) class IIB in Atlantic salmon (Salmo salar) for 
      survival after challenge with a highly virulent bacterial pathogen. Forty 
      juvenile full siblings from each of 120 families were infected with the bacterium 
      Aeromonas salmonicida, which causes high mortality in salmon due to furunculosis. 
      Fishes from high-resistance (HR, < 35% mortality) and low-resistance (L,R, > 80% 
      mortality) families were screened for their MHC class IIB genotypes using the 
      denaturing gradient gel electrophoresis (DGGE) technique. The exon 2 sequences, 
      encoding the major part of the peptide-binding region, were established for each 
      DGGE fragment. One allele, e, containing a missense single base substitution was 
      significantly more prevalent in HR families than in LR families. An odds-ratio 
      test showed that broods carrying this allele had a 12-fold higher chance of being 
      HR than broods without the e allele. A second allele, i, showed significantly 
      higher frequencies in uninfected and surviving individuals than in infected dead 
      individuals. A third allele, j, tended to more prevalent both in LR families and 
      in individuals that had died of the infection. There was no correlation between 
      MHC heterozygosity and resistance to A. salmonicida. Our results support the 
      hypothesis that MHC polymorphism is maintained through pathogen-driven selection 
      acting by means of frequency-dependent selection rather than heterozygous 
      advantage.
FAU - Langefors, A
AU  - Langefors A
AD  - Department of Animal Ecology, Lund University, Sweden. 
      asa.langefors@zooekol.lu.se
FAU - Lohm, J
AU  - Lohm J
FAU - Grahn, M
AU  - Grahn M
FAU - Andersen, O
AU  - Andersen O
FAU - von Schantz, T
AU  - von Schantz T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Proc Biol Sci
JT  - Proceedings. Biological sciences
JID - 101245157
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Aeromonas/*pathogenicity
MH  - Alleles
MH  - Animals
MH  - Base Sequence
MH  - DNA/genetics
MH  - Exons
MH  - *Genes, MHC Class II
MH  - Genetic Variation
MH  - Molecular Sequence Data
MH  - Salmo salar/*genetics/*immunology/microbiology
PMC - PMC1088630
EDAT- 2001/04/12 10:00
MHDA- 2001/06/29 10:01
PMCR- 2003/03/07
CRDT- 2001/04/12 10:00
PHST- 2001/04/12 10:00 [pubmed]
PHST- 2001/06/29 10:01 [medline]
PHST- 2001/04/12 10:00 [entrez]
PHST- 2003/03/07 00:00 [pmc-release]
AID - 10.1098/rspb.2000.1378 [doi]
PST - ppublish
SO  - Proc Biol Sci. 2001 Mar 7;268(1466):479-85. doi: 10.1098/rspb.2000.1378.