PMID- 11297674
OWN - NLM
STAT- MEDLINE
DCOM- 20010524
LR  - 20220316
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 82
IP  - Pt 5
DP  - 2001 May
TI  - Identification of an immunodominant epitope in the C terminus of glycoprotein 5 
      of porcine reproductive and respiratory syndrome virus.
PG  - 995-999
LID - 10.1099/0022-1317-82-5-995 [doi]
AB  - Glycoprotein 5 (GP(5)) is the major glycoprotein of porcine reproductive and 
      respiratory syndrome virus (PRRSV). Expression of GP(5) has been improved by 
      removing the transmembrane regions. Vectors were constructed encoding complete 
      GP(5) plus three mutants: GP(5) Ns (residues 28--201), GP(5)[30--67] (residues 
      30--67) and GP(5)[30--201] (residues 30--67/130--201). The three deletion mutants 
      were expressed at levels 20--30 times higher than complete GP(5). GP(5)[30--201] 
      was well recognized in ELISA or immunoblotting by a collection of pig sera. All 
      the fragments were tested for the generation of MAbs, but only the 
      polyhistidine-tagged fragment GP(5)[30--201]H elicited an antibody response 
      sufficient to produce MABS: The two MAbs were positive for PRRSV in ELISA and 
      immunoblotting, but negative for virus neutralization. MAb 4BE12 reacted with 
      residues 130--170 and MAb 3AH9 recognized residues 170--201. This region was 
      recognized strongly in immunoblotting by a collection of infected-pig sera. These 
      results indicate diagnostic potential for this epitope.
FAU - Rodriguez, Maria Jose
AU  - Rodriguez MJ
AD  - INGENASA, Hermanos Garcia Noblejas 41, 28037 Madrid, Spain1.
FAU - Sarraseca, Javier
AU  - Sarraseca J
AD  - INGENASA, Hermanos Garcia Noblejas 41, 28037 Madrid, Spain1.
FAU - Fominaya, Jesus
AU  - Fominaya J
AD  - INGENASA, Hermanos Garcia Noblejas 41, 28037 Madrid, Spain1.
FAU - Cortes, Elena
AU  - Cortes E
AD  - INGENASA, Hermanos Garcia Noblejas 41, 28037 Madrid, Spain1.
FAU - Sanz, Antonio
AU  - Sanz A
AD  - INGENASA, Hermanos Garcia Noblejas 41, 28037 Madrid, Spain1.
FAU - Casal, J Ignacio
AU  - Casal JI
AD  - INGENASA, Hermanos Garcia Noblejas 41, 28037 Madrid, Spain1.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (Epitopes, B-Lymphocyte)
RN  - 0 (Immunodominant Epitopes)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Antibodies, Viral/blood/immunology
MH  - Antigens, Viral/genetics/*immunology
MH  - Epitopes, B-Lymphocyte/genetics/*immunology
MH  - Immunodominant Epitopes/genetics/*immunology
MH  - Membrane Glycoproteins/genetics/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mutagenesis
MH  - Neutralization Tests
MH  - Porcine Reproductive and Respiratory Syndrome/blood/immunology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*immunology
MH  - Swine
MH  - Viral Envelope Proteins/genetics/*immunology
EDAT- 2001/04/12 10:00
MHDA- 2001/05/26 10:01
CRDT- 2001/04/12 10:00
PHST- 2001/04/12 10:00 [pubmed]
PHST- 2001/05/26 10:01 [medline]
PHST- 2001/04/12 10:00 [entrez]
AID - 10.1099/0022-1317-82-5-995 [doi]
PST - ppublish
SO  - J Gen Virol. 2001 May;82(Pt 5):995-999. doi: 10.1099/0022-1317-82-5-995.