PMID- 11297762
OWN - NLM
STAT- MEDLINE
DCOM- 20010426
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 125
IP  - 1
DP  - 2001 Feb
TI  - Tetrasomy 8 is associated with a major cellular proliferative advantage and a 
      poor prognosis. two cases of myeloid hematologic disorders and review of the 
      literature.
PG  - 14-20
AB  - We report two cases of acute myeloid leukemia (AML) with tetrasomy 8 detected in 
      patients' bone marrow samples using chromosome GTG-banding, fluorescence in situ 
      hybridization (FISH) and primed in situ labeling (PRINS) techniques. Case 1 was a 
      myelodysplastic syndrome (MDS) in transition to AML-M4 and case 2 was an AML-M2. 
      In case 1, the tetrasomy 8 was found in 40% of metaphase cells and constituted 
      the only chromosome abnormality. In case 2, it was accompanied by a double Ph, 
      trisomy 18 and disomy Y and was found in 68% of metaphase cells. However, FISH 
      and PRINS techniques revealed the coexistence of tetrasomy 8 and trisomy 8 in 
      interphase nuclei of both cases. When the proportion of cells with tetrasomy 8 
      was compared between metaphases and interphase nuclei, it showed a much higher 
      percentage of cells with tetrasomy 8 in metaphases than in interphase nuclei. 
      Moreover, in case 2, although multi-PRINS and FISH-PRINS techniques showed other 
      populations of interphase nuclei with different combinations of chromosome 
      anomalies with respect to the copy numbers for chromosomes 8, 18, Y and Ph, only 
      cells that contained either a single Ph or tetrasomy 8 plus trisomy 18, disomy Y, 
      and double Ph could be seen in metaphases. This strongly suggests that tetrasomy 
      8 confers a higher proliferative advantage to cells. Our cases also show that the 
      tetrasomy 8 is associated with a poor prognosis.
FAU - Yan, J
AU  - Yan J
AD  - Division of Pathology, Department of Medical Biology, Faculty of Medicine, Laval 
      University, and Unite de Recherche en Genetique Humaine et Moleculaire, Hopital 
      Saint-Francois d'Assise, CHUQ, 10 de l'Espinay, G1L 3L5, Quebec, Canada.
FAU - Marceau, D
AU  - Marceau D
FAU - Drouin, R
AU  - Drouin R
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA Primers)
SB  - IM
MH  - Aged
MH  - Base Sequence
MH  - *Chromosome Aberrations
MH  - *Chromosome Disorders
MH  - *Chromosomes, Human, Pair 8
MH  - DNA Primers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Myeloid, Acute/diagnosis/drug therapy/*genetics
MH  - Male
MH  - Middle Aged
RF  - 29
EDAT- 2001/04/12 10:00
MHDA- 2001/05/01 10:01
CRDT- 2001/04/12 10:00
PHST- 2001/04/12 10:00 [pubmed]
PHST- 2001/05/01 10:01 [medline]
PHST- 2001/04/12 10:00 [entrez]
AID - S0165-4608(00)00352-6 [pii]
AID - 10.1016/s0165-4608(00)00352-6 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2001 Feb;125(1):14-20. doi: 
      10.1016/s0165-4608(00)00352-6.