PMID- 11298057
OWN - NLM
STAT- MEDLINE
DCOM- 20010503
LR  - 20190906
IS  - 1464-4096 (Print)
IS  - 1464-4096 (Linking)
VI  - 87
IP  - 6
DP  - 2001 Apr
TI  - Ontogeny of bladder agenesis in rats induced by adriamycin.
PG  - 556-61
AB  - OBJECTIVES: To determine the anatomical steps leading to bladder agenesis in rats 
      prenatally exposed as fetuses on gestational days (GD) 6-9 to adriamycin. 
      MATERIALS AND METHODS: Timed-pregnant Sprague-Dawley rats were injected 
      intraperitoneally with adriamycin at 2 mg/kg (n = 28) on GD 6-9 (vaginal plug = 
      day 0). The control group (n = 21) received saline. Fetuses were harvested on GD 
      10, 11, 12, 13, 14, 15 and 16. Serial paraffin sections were prepared from a 
      minimum of 10 experimental and five control fetuses at each gestational age, and 
      stained with either trichrome or haematoxylin and eosin, and examined by light 
      microscopy. RESULTS: In the control group the urorectal septum first became 
      visible and the mesonephric ducts apparently abutting the anterior cloaca on GD 
      12. The presumptive urinary bladder was clearly defined on GD 14. On GD 15, the 
      common excretory ducts became incorporated into the newly formed urogenital sinus 
      and the ureters opened into the bladder. In the treated animals, beginning on GD 
      11, the undivided cloaca was noticeably smaller and by GD 13-14, the vesical 
      extension of the urogenital sinus was conspicuously absent. Instead, opposite 
      ureters joined to drain directly into the proximal blind-ending urethra or the 
      persistent distal urogenital sinus. CONCLUSIONS: Prenatal exposure of rat fetuses 
      to adriamycin resulted in primary agenesis rather than secondary resorption of 
      the bladder. The ontogeny showed that the mechanism underpinning bladder 
      development is unique and is under the influence of factors that can be targeted 
      by adriamycin. Further work will elucidate the unique nature of bladder 
      organogenesis and should have important applications in future research into 
      artificial bladders.
FAU - Liu, M I
AU  - Liu MI
AD  - F. Douglas Stephens Surgical Research Laboratory, Murdoch Children's Hospital 
      Research Institute, Melbourne, Australia.
FAU - Hutson, J M
AU  - Hutson JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - BJU Int
JT  - BJU international
JID - 100886721
RN  - 0 (Antineoplastic Agents)
RN  - 80168379AG (Doxorubicin)
SB  - IM
MH  - Abnormalities, Drug-Induced/*embryology/pathology
MH  - Animals
MH  - Antineoplastic Agents/*toxicity
MH  - Doxorubicin/*toxicity
MH  - Female
MH  - Pregnancy
MH  - Prenatal Exposure Delayed Effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Urinary Bladder/*abnormalities/embryology
EDAT- 2001/04/12 10:00
MHDA- 2001/05/05 10:01
CRDT- 2001/04/12 10:00
PHST- 2001/04/12 10:00 [pubmed]
PHST- 2001/05/05 10:01 [medline]
PHST- 2001/04/12 10:00 [entrez]
AID - bju087 [pii]
AID - 10.1046/j.1464-410x.2001.00087.x [doi]
PST - ppublish
SO  - BJU Int. 2001 Apr;87(6):556-61. doi: 10.1046/j.1464-410x.2001.00087.x.