PMID- 11298325
OWN - NLM
STAT- MEDLINE
DCOM- 20010510
LR  - 20191104
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 31
IP  - 4
DP  - 2001 Apr
TI  - Interleukin-18 enhances the production of interleukin-8 by eosinophils.
PG  - 1010-6
AB  - Interleukin-18 (IL-18), a proinflammatory cytokine, leads to IFN-gamma production 
      by NK or T cells, induces Th1 differentiation and suppresses IgE synthesis by B 
      cells when acting on responding cells together with IL-12. IL-18 also exhibits 
      biological activities related to allergic inflammation such as histamine or IL-4 
      release from basophils and accumulation of eosinophils in localized lesions in 
      allergic model mice. In this study, Reverse transcription (RT)-PCR analysis 
      revealed that IL-18 receptor alpha chain mRNA was expressed in both freshly 
      prepared eosinophils and two eosinophilic cell lines (YY-1 and EoL-1 cells). Flow 
      cytometry and RT-PCR analyses revealed that the treatment of YY-1 cells with 
      n-butyric acid promoted cell maturation and caused an enhancement of IL-18 
      receptor alpha chain expression. IL-18 had little effect on the survival of 
      peripheral eosinophils, but it dose-dependently augmented IL-8 synthesis by YY-1 
      cells. In addition, IL-18-mediated up-regulation of IL-8 expression in 
      eosinophils from a patient suffering from hyper-eosinophilic syndrome was 
      confirmed. Our findings using peripheral blood eosinophils and eosinophilic cell 
      line suggest the functional importance of IL-18 in the induction of IL-8 and a 
      potential proinflammatory role in allergy.
FAU - Wang, W
AU  - Wang W
AD  - Department of Internal Medicine III, Osaka University Medical School, Osaka, 
      Japan.
FAU - Tanaka, T
AU  - Tanaka T
FAU - Okamura, H
AU  - Okamura H
FAU - Sugita, M
AU  - Sugita M
FAU - Higa, S
AU  - Higa S
FAU - Kishimoto, T
AU  - Kishimoto T
FAU - Suemura, M
AU  - Suemura M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (IL18R1 protein, human)
RN  - 0 (Interleukin-18)
RN  - 0 (Interleukin-18 Receptor alpha Subunit)
RN  - 0 (Interleukin-8)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Interleukin)
RN  - 0 (Receptors, Interleukin-18)
RN  - 107-92-6 (Butyric Acid)
SB  - IM
MH  - Butyric Acid/pharmacology
MH  - Cell Differentiation/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Drug
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Eosinophils/*drug effects/*metabolism/pathology
MH  - Flow Cytometry
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Hypereosinophilic Syndrome/metabolism/pathology
MH  - Interleukin-18/*pharmacology
MH  - Interleukin-18 Receptor alpha Subunit
MH  - Interleukin-8/*biosynthesis/genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - Receptors, Interleukin/chemistry/genetics/metabolism
MH  - Receptors, Interleukin-18
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Cells, Cultured
EDAT- 2001/04/12 10:00
MHDA- 2001/05/22 10:01
CRDT- 2001/04/12 10:00
PHST- 2001/04/12 10:00 [pubmed]
PHST- 2001/05/22 10:01 [medline]
PHST- 2001/04/12 10:00 [entrez]
AID - 10.1002/1521-4141(200104)31:4<1010::AID-IMMU1010>3.0.CO;2-8 [pii]
AID - 10.1002/1521-4141(200104)31:4<1010::aid-immu1010>3.0.co;2-8 [doi]
PST - ppublish
SO  - Eur J Immunol. 2001 Apr;31(4):1010-6. doi: 
      10.1002/1521-4141(200104)31:4<1010::aid-immu1010>3.0.co;2-8.