PMID- 11298800 OWN - NLM STAT- MEDLINE DCOM- 20010621 LR - 20220309 IS - 0953-816X (Print) IS - 0953-816X (Linking) VI - 13 IP - 7 DP - 2001 Apr TI - PSA-NCAM modulates BDNF-dependent survival and differentiation of cortical neurons. PG - 1391-402 AB - We show that the loss or inactivation of the polysialic acid (PSA) tail of neural cell adhesion molecule (NCAM) on rat cortical neurons in culture leads to reduced differentiation and survival. The mechanism by which this negative effect is mediated appears to involve the neuronal response to brain-derived neurotrophic factor (BDNF): (i) in the absence of PSA or in the presence of excess free PSA added to the culture medium, BDNF-induced cell signalling is reduced; (ii) the addition of exogenous BDNF to the medium reverses the effect of PSA loss or inactivation. These data suggest that PSA-NCAM, previously shown to modulate cell migration and plasticity, is needed for an adequate sensitivity of neurons to BDNF. FAU - Vutskits, L AU - Vutskits L AD - Department of Morphology, University of Geneva Medical School, 1 rue Michel Servet, CH-1211 Geneva 4, Switzerland. FAU - Djebbara-Hannas, Z AU - Djebbara-Hannas Z FAU - Zhang, H AU - Zhang H FAU - Paccaud, J P AU - Paccaud JP FAU - Durbec, P AU - Durbec P FAU - Rougon, G AU - Rougon G FAU - Muller, D AU - Muller D FAU - Kiss, J Z AU - Kiss JZ LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - France TA - Eur J Neurosci JT - The European journal of neuroscience JID - 8918110 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Neural Cell Adhesion Molecule L1) RN - 0 (Neural Cell Adhesion Molecules) RN - 0 (Recombinant Fusion Proteins) RN - 0 (Sialic Acids) RN - 0 (polysialic acid) RN - 0 (polysialyl neural cell adhesion molecule) RN - EC 2.7.10.1 (Receptor, trkB) RN - EC 3.2.1.- (Glycoside Hydrolases) RN - EC 3.2.1.129 (endo-alpha-sialidase) SB - IM MH - Animals MH - Animals, Newborn MH - Brain-Derived Neurotrophic Factor/*pharmacology MH - Cell Death/drug effects/physiology MH - Cell Differentiation/drug effects/physiology MH - Cell Survival/drug effects/physiology MH - Cerebral Cortex/*cytology MH - Glycoside Hydrolases/metabolism MH - Humans MH - *Neural Cell Adhesion Molecule L1 MH - Neural Cell Adhesion Molecules/genetics/*metabolism/pharmacology MH - Neurons/*cytology/metabolism MH - Rats MH - Rats, Sprague-Dawley MH - Receptor, trkB/metabolism MH - Recombinant Fusion Proteins/genetics/metabolism MH - Rhabdomyosarcoma MH - Sialic Acids/genetics/*metabolism/pharmacology MH - Signal Transduction/physiology MH - Transfection MH - Tumor Cells, Cultured EDAT- 2001/04/12 10:00 MHDA- 2001/06/22 10:01 CRDT- 2001/04/12 10:00 PHST- 2001/04/12 10:00 [pubmed] PHST- 2001/06/22 10:01 [medline] PHST- 2001/04/12 10:00 [entrez] AID - ejn1516 [pii] AID - 10.1046/j.0953-816x.2001.01516.x [doi] PST - ppublish SO - Eur J Neurosci. 2001 Apr;13(7):1391-402. doi: 10.1046/j.0953-816x.2001.01516.x.