PMID- 11300507
OWN - NLM
STAT- MEDLINE
DCOM- 20010621
LR  - 20190513
IS  - 0146-4760 (Print)
IS  - 0146-4760 (Linking)
VI  - 25
IP  - 2
DP  - 2001 Mar
TI  - Sweat testing of MDMA with the Drugwipe analytical device: a controlled study 
      with two volunteers.
PG  - 144-6
AB  - Rapid on-site tests for the analysis of drugs of abuse in unconventional 
      specimens (e.g., sweat) have recently been developed. Two healthy volunteers 
      familiar with the effects of methylenedioxymethamphetamine (MDMA) were given 100 
      mg of the drug as a single oral dose. MDMA and its main metabolite 
      4-hydroxy-3-methoxymethamphetamine (HMMA) were determined in plasma and urine by 
      gas chromatography-mass spectrometry (GC-MS). MDMA was also investigated in sweat 
      with the Drugwipe (an immunochemical strip test). Subjects' armpits were swabbed 
      for 10 s at 0 time (predose) and at 2, 6, 8, 12, and 24 h after MDMA 
      administration. MDMA consumption could be detected using Drugwipe at 2 h and for 
      as long as 12 h after drug administration. However, in one of the volunteers, a 
      faint color change appeared at 0 time, when plasma and urine tested negative for 
      MDMA and did not disappear even 48 h later. Plasma concentrations of MDMA and 
      HMMA measured by GC-MS peaked at 2-4 h, and values greater than 20 ng/mL for MDMA 
      and of 40 ng/mL for HMMA were still detected at 24 h. Urine tested positive by 
      GC-MS for MDMA and HMMA in the 48-h collection period. These findings 
      preliminarily support sweat testing with Drugwipe for monitoring MDMA use.
FAU - Pacifici, R
AU  - Pacifici R
AD  - Istituto Superiore di Sanita, Roma, Italy.
FAU - Farre, M
AU  - Farre M
FAU - Pichini, S
AU  - Pichini S
FAU - Ortuno, J
AU  - Ortuno J
FAU - Roset, P N
AU  - Roset PN
FAU - Zuccaro, P
AU  - Zuccaro P
FAU - Segura, J
AU  - Segura J
FAU - de la Torre, R
AU  - de la Torre R
LA  - eng
PT  - Clinical Trial
PT  - Controlled Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - 0 (Lactates)
RN  - 0 (Reagent Strips)
RN  - 515-30-0 (atrolactic acid)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Humans
MH  - Immunochemistry/methods
MH  - Lactates/analysis
MH  - Male
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*analysis/pharmacokinetics
MH  - *Reagent Strips
MH  - Substance Abuse Detection/instrumentation/*methods
MH  - Sweat/*chemistry
MH  - Time Factors
EDAT- 2001/04/13 10:00
MHDA- 2001/06/22 10:01
CRDT- 2001/04/13 10:00
PHST- 2001/04/13 10:00 [pubmed]
PHST- 2001/06/22 10:01 [medline]
PHST- 2001/04/13 10:00 [entrez]
AID - 10.1093/jat/25.2.144 [doi]
PST - ppublish
SO  - J Anal Toxicol. 2001 Mar;25(2):144-6. doi: 10.1093/jat/25.2.144.