PMID- 11301190
OWN - NLM
STAT- MEDLINE
DCOM- 20010503
LR  - 20191025
IS  - 0301-472X (Print)
IS  - 0301-472X (Linking)
VI  - 29
IP  - 4
DP  - 2001 Apr
TI  - T-cell factor-1 expression during human natural killer cell development and in 
      circulating CD56(+) bright natural killer cells.
PG  - 499-506
AB  - Transcription factors are essential to govern differentiation along the lymphoid 
      lineage from uncommitted hematopoietic stem cells. Although many of these 
      transcription factors have putative roles based on murine knockout experiments, 
      their function in human lymphoid development is less known and was studied 
      further. Transcription factor expression in fresh and cultured adult human bone 
      marrow and umbilical cord blood progenitors was evaluated. We found that fresh 
      CD34(+)Lin(-) cells that are human leukocyte antigen (HLA)-DR(-) or CD38(-) 
      constitutively express GATA-3 but not T-cell factor-1 (TCF-1) or Id-3. Culture 
      with the murine fetal liver cell line AFT024 and defined cytokines was capable of 
      inducing TCF-1 mRNA. However, no T-cell receptor gene rearrangement was 
      identified in cultured progeny. Id-3, a basic helix loop helix factor with 
      dominant negative function for T-cell differentiation transcription factors, also 
      was upregulated and may explain unsuccessful T-cell maturation. To better 
      understand the developmental link between natural killer (NK) cells derived from 
      progenitors, we studied NK cell subsets circulating in blood. CD56(+bright), but 
      not CD56(+dim), NK cells constitutively express TCF-1 by reverse transcriptase 
      polymerase chain reaction and Western blot analysis. The TCF-1 isoform found in 
      CD56(+bright) cells, which express lectin but not immunoglobulin class I 
      recognizing inhibitory receptors, was identical to that induced in NK cell 
      differentiation culture and was distinctly different from isoforms in T cells. 
      These results suggest that TCF-1 does not target human killer immunoglobulin 
      receptor genes, TCF-1 is uniquely expressed in circulating CD56(+bright) NK 
      cells, and specific TCF-1 isoforms may play an important role in regulating NK 
      differentiation from a common NK/T-cell progenitor.
FAU - Toor, A A
AU  - Toor AA
AD  - Department of Medicine, University of Minnesota Cancer Center, Harvard Street at 
      East River Road, Minneapolis, MN 55455, USA.
FAU - Lund, T C
AU  - Lund TC
FAU - Miller, J S
AU  - Miller JS
LA  - eng
GR  - M01-RR00400/RR/NCRR NIH HHS/United States
GR  - P01-CA-65493/CA/NCI NIH HHS/United States
GR  - R01-HL-55417/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Exp Hematol
JT  - Experimental hematology
JID - 0402313
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, CD34)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (CD56 Antigen)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Hepatocyte Nuclear Factor 1-alpha)
RN  - 0 (Hnf1a protein, mouse)
RN  - 0 (Inhibitor of Differentiation Proteins)
RN  - 0 (Lymphoid Enhancer-Binding Factor 1)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Mitogen)
RN  - 0 (T Cell Transcription Factor 1)
RN  - 0 (TCF7 protein, human)
RN  - 0 (Transcription Factors)
RN  - 147785-34-0 (ID3 protein, human)
RN  - EC 3.2.2.5 (ADP-ribosyl Cyclase)
RN  - EC 3.2.2.5 (CD38 protein, human)
RN  - EC 3.2.2.5 (Cd38 protein, mouse)
RN  - EC 3.2.2.5 (NAD+ Nucleosidase)
RN  - EC 3.2.2.6 (ADP-ribosyl Cyclase 1)
SB  - IM
MH  - ADP-ribosyl Cyclase
MH  - ADP-ribosyl Cyclase 1
MH  - Animals
MH  - *Antigens, CD
MH  - Antigens, CD34/analysis
MH  - Antigens, Differentiation/analysis
MH  - Blotting, Western
MH  - CD56 Antigen/*analysis
MH  - Cells, Cultured
MH  - DNA-Binding Proteins/*genetics
MH  - *Gene Expression
MH  - HLA-DR Antigens/analysis
MH  - Helix-Loop-Helix Motifs
MH  - Hematopoietic Stem Cells/immunology/physiology
MH  - Hepatocyte Nuclear Factor 1-alpha
MH  - Humans
MH  - Inhibitor of Differentiation Proteins
MH  - Killer Cells, Natural/immunology/*physiology
MH  - Lymphoid Enhancer-Binding Factor 1
MH  - Membrane Glycoproteins
MH  - Mice
MH  - NAD+ Nucleosidase/analysis
MH  - *Neoplasm Proteins
MH  - RNA, Messenger/analysis
MH  - Receptors, Mitogen/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - T Cell Transcription Factor 1
MH  - Transcription Factors/analysis/*genetics
EDAT- 2001/04/13 10:00
MHDA- 2001/05/05 10:01
CRDT- 2001/04/13 10:00
PHST- 2001/04/13 10:00 [pubmed]
PHST- 2001/05/05 10:01 [medline]
PHST- 2001/04/13 10:00 [entrez]
AID - S0301-472X(00)00680-9 [pii]
AID - 10.1016/s0301-472x(00)00680-9 [doi]
PST - ppublish
SO  - Exp Hematol. 2001 Apr;29(4):499-506. doi: 10.1016/s0301-472x(00)00680-9.