PMID- 11302744
OWN - NLM
STAT- MEDLINE
DCOM- 20010517
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 282
IP  - 5
DP  - 2001 Apr 20
TI  - Menin, a gene product responsible for multiple endocrine neoplasia type 1, 
      interacts with the putative tumor metastasis suppressor nm23.
PG  - 1206-10
AB  - Although the gene responsible for multiple endocrine neoplasia type 1 (MEN1) has 
      been identified, the function of its gene product, menin, is unknown. To examine 
      the biological role of the MEN1 gene, we searched for associated proteins with a 
      yeast two-hybrid system using the MEN1 cDNA fragment as bait. On screening a rat 
      fetal brain embryonic day 17 library, in which a high level of MEN1 expression 
      was detected, we identified a putative tumor metastasis suppressor 
      nm23/nucleoside diphosphate (NDP) kinase as an associated protein. This finding 
      was confirmed by in vitro interaction assays based on glutathione S-transferase 
      pull down experiments. The association required almost the entire menin protein, 
      and several missense MEN1 mutations reported in MEN1 patients caused a loss of 
      the binding activity for nm23. This result suggests that this interaction may 
      play important roles in the biological functions of the menin protein, including 
      tumor suppressor activity.
CI  - Copyright 2001 Academic Press.
FAU - Ohkura, N
AU  - Ohkura N
AD  - Growth Factor Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, 
      Chuo-ku, Tokyo, 104-0045, Japan. nohkura@gan2.ncc.go.jp
FAU - Kishi, M
AU  - Kishi M
FAU - Tsukada, T
AU  - Tsukada T
FAU - Yamaguchi, K
AU  - Yamaguchi K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (MEN1 protein, human)
RN  - 0 (NM23 Nucleoside Diphosphate Kinases)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Sulfur Radioisotopes)
RN  - 0 (Transcription Factors)
RN  - AE28F7PNPL (Methionine)
RN  - EC 2.5.1.18 (Glutathione Transferase)
RN  - EC 2.7.4.6 (NME1 protein, human)
RN  - EC 2.7.4.6 (Nucleoside-Diphosphate Kinase)
RN  - EC 3.6.5.2 (Monomeric GTP-Binding Proteins)
SB  - IM
MH  - Animals
MH  - COS Cells
MH  - Cell-Free System
MH  - Genes, Tumor Suppressor/genetics
MH  - Glutathione Transferase/genetics
MH  - Methionine/metabolism
MH  - Monomeric GTP-Binding Proteins/*metabolism
MH  - Multiple Endocrine Neoplasia Type 1/*etiology/genetics
MH  - Mutation, Missense/genetics
MH  - NM23 Nucleoside Diphosphate Kinases
MH  - Neoplasm Proteins/genetics/*metabolism
MH  - *Nucleoside-Diphosphate Kinase
MH  - Protein Binding/physiology
MH  - Protein Structure, Tertiary/physiology
MH  - *Proto-Oncogene Proteins
MH  - Rats
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Sulfur Radioisotopes
MH  - Transcription Factors/*metabolism
MH  - Two-Hybrid System Techniques
EDAT- 2001/04/17 10:00
MHDA- 2001/05/18 10:01
CRDT- 2001/04/17 10:00
PHST- 2001/04/17 10:00 [pubmed]
PHST- 2001/05/18 10:01 [medline]
PHST- 2001/04/17 10:00 [entrez]
AID - S0006-291X(01)94723-0 [pii]
AID - 10.1006/bbrc.2001.4723 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2001 Apr 20;282(5):1206-10. doi: 
      10.1006/bbrc.2001.4723.