PMID- 11304717
OWN - NLM
STAT- MEDLINE
DCOM- 20010628
LR  - 20190906
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 433
IP  - 4
DP  - 2001 May 14
TI  - Target-derived BDNF (brain-derived neurotrophic factor) is essential for the 
      survival of developing neurons in the isthmo-optic nucleus.
PG  - 550-64
AB  - Neurons in the peripheral nervous system depend on single neurotrophic factors, 
      whereas those in the brain are thought to utilize many different trophic factors. 
      This study examined whether some neurons in the brain critically depend on a 
      single trophic factor during development. Neurons in the isthmo-optic nucleus 
      (ION) of chick embryos respond to exogenous brain-derived neurotrophic factor 
      (BDNF). Relatively high concentrations of endogenous BDNF were present in the ION 
      of 14-18-day-old chick embryos. ION target cells in the retina were immunolabeled 
      for BDNF but showed surprisingly low levels of BDNF mRNA. These data suggest that 
      ION target cells derive some BDNF from other retinal sources. No BDNF mRNA was 
      detected in the ION itself. ION neurons had a very efficient retrograde transport 
      system for BDNF and exogenous BDNF arrived in the ION intact. When the ION was 
      deprived of endogenous trkB ligands by injection of trkB fusion proteins in the 
      eye, cell death of ION neurons was enhanced, and this effect was mimicked by 
      BDNF-specific blocking antibodies in the eye. TrkB fusion proteins in the retina 
      induced cell death of ION neurons prior to visible effects on ION target cells in 
      the retina. Immunolabel for endogenous BDNF was sparse in pyknotic ION neurons, 
      suggesting that ION neurons with low BDNF content were eliminated by apoptosis. 
      These data show that BDNF is an essential target-derived trophic factor for 
      developing ION neurons and thereby validate the neurotrophic hypothesis for at 
      least one neuronal population in the brain.
CI  - Copyright 2001 Wiley-Liss, Inc.
FAU - Von Bartheld, C S
AU  - Von Bartheld CS
AD  - Department of Physiology and Cell Biology, University of Nevada School of 
      Medicine, Reno, Nevada 89557, USA. chrisvb@physio.unr.edu
FAU - Johnson, J E
AU  - Johnson JE
LA  - eng
GR  - EY 11127/EY/NEI NIH HHS/United States
GR  - NS 35931/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Antibodies)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (RNA, Messenger)
RN  - EC 1.6.99.1 (NADPH Dehydrogenase)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - P658DCA9XD (neurotrophin 4)
SB  - IM
MH  - Animals
MH  - Antibodies/pharmacology
MH  - Brain/cytology/*embryology/metabolism
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism/pharmacology
MH  - Cell Communication/drug effects/physiology
MH  - Cell Death/drug effects/physiology
MH  - Cell Differentiation/drug effects/*physiology
MH  - Cell Survival/drug effects/*physiology
MH  - Chick Embryo/cytology/*embryology/metabolism
MH  - Immunohistochemistry
MH  - NADPH Dehydrogenase/metabolism
MH  - Nerve Growth Factors/metabolism/pharmacology
MH  - Neurons/cytology/drug effects/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Receptor, trkB/metabolism
MH  - Retina/cytology/embryology/metabolism
MH  - Visual Pathways/cytology/*embryology/metabolism
EDAT- 2001/04/17 10:00
MHDA- 2001/06/29 10:01
CRDT- 2001/04/17 10:00
PHST- 2001/04/17 10:00 [pubmed]
PHST- 2001/06/29 10:01 [medline]
PHST- 2001/04/17 10:00 [entrez]
AID - 10.1002/cne.1159 [doi]
PST - ppublish
SO  - J Comp Neurol. 2001 May 14;433(4):550-64. doi: 10.1002/cne.1159.