PMID- 11304727
OWN - NLM
STAT- MEDLINE
DCOM- 20010503
LR  - 20061115
IS  - 0270-4137 (Print)
IS  - 0270-4137 (Linking)
VI  - 47
IP  - 1
DP  - 2001 Apr
TI  - High-grade prostate intraepithelial neoplasia shares cytogenetic alterations with 
      invasive prostate cancer.
PG  - 29-35
AB  - BACKGROUND: High-grade prostate intraepithelial neoplasia (PIN) is the most 
      likely precursor of prostate adenocarcinoma. However, the relationship between 
      this lesion and prostate cancer has not yet been established. The detection of 
      cytogenetic changes in the lesions prior to prostate adenocarcinoma would be 
      useful in demonstrating such a pathogenic relationship. METHODS: Twenty eight 
      high-grade PIN cases were found among 57 specimens of radical prostatectomy 
      performed for clinically localized prostate cancer. Fluorescence in situ 
      hybridization (FISH) analysis using centromeric probes to enumerate chromosomes 
      7, 8, 10, and 12 was performed to study the numerical chromosome alterations. 
      FISH analysis was carried out over isolated nuclei obtained from high-grade PIN 
      areas and prostate cancer foci in the same prostatectomy specimen. RESULTS: Of 
      the 28 suitable cases it was possible to complete the study in 26 tumor and 20 
      PIN areas. The remaining cases were excluded because of insufficient tissue or 
      poor preservation. Cytogenetic alterations (aneuploidy) were found in 16 of the 
      26 (62%) tumors studied. The most frequent chromosome alteration was trisomy 7, 
      detected in 12 (75%) aneuploid tumors, followed by monosomy 8 present in 5 (31%) 
      aneuploid tumors. Trisomy 7 was also the most frequent isolated chromosome 
      alteration since it was detected in 7 (44%) tumors. Thirteen of 20 (65%) PIN 
      cases were aneuploid when studied by FISH. Trisomy 7, trisomy 8, and monosomy 8 
      were the most common cytogenetic alterations in the 20 PIN areas studied, being 
      observed in nine (45%), six (30%), and four (20%) cases, respectively. FISH 
      analysis showed a high correlation (75% cases) in ploidy and pattern of 
      cytogenetic alterations between high-grade PIN areas and the paired prostate 
      cancer focus in the same specimen. CONCLUSIONS: The above results show a 
      cytogenetic link between high-grade PIN and prostate cancer, suggesting that the 
      former could be an early form of prostate cancer.
FAU - Alcaraz, A
AU  - Alcaraz A
AD  - Department of Urology and Renal Transplantation, Institut de Investigacions 
      Biomediques Agusti Pi i Sunyer (IDIBAPS), Hospital Clinic, Barcelona, Spain. 
      aalcaraz@clinic.ub.es
FAU - Barranco, M A
AU  - Barranco MA
FAU - Corral, J M
AU  - Corral JM
FAU - Ribal, M J
AU  - Ribal MJ
FAU - Carrio, A
AU  - Carrio A
FAU - Mallofre, C
AU  - Mallofre C
FAU - Llopis, J
AU  - Llopis J
FAU - Cetina, A
AU  - Cetina A
FAU - Alvarez-Vijande, R
AU  - Alvarez-Vijande R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Prostate
JT  - The Prostate
JID - 8101368
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adenocarcinoma/*genetics/*pathology
MH  - Cell Nucleus/pathology
MH  - Cytogenetic Analysis
MH  - DNA, Neoplasm/analysis
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Neoplasm Invasiveness
MH  - Predictive Value of Tests
MH  - Prostatic Intraepithelial Neoplasia/*genetics/*pathology
MH  - Prostatic Neoplasms/*genetics/*pathology
EDAT- 2001/04/17 10:00
MHDA- 2001/05/05 10:01
CRDT- 2001/04/17 10:00
PHST- 2001/04/17 10:00 [pubmed]
PHST- 2001/05/05 10:01 [medline]
PHST- 2001/04/17 10:00 [entrez]
AID - 10.1002/pros.1044 [doi]
PST - ppublish
SO  - Prostate. 2001 Apr;47(1):29-35. doi: 10.1002/pros.1044.