PMID- 11304855
OWN - NLM
STAT- MEDLINE
DCOM- 20010426
LR  - 20220318
IS  - 0021-9746 (Print)
IS  - 1472-4146 (Electronic)
IS  - 0021-9746 (Linking)
VI  - 54
IP  - 4
DP  - 2001 Apr
TI  - Influence of smoking and alcohol on gastric chemokine mRNA expression in patients 
      with Helicobacter pylori infection.
PG  - 332-4
AB  - AIM: Chemokines that play a primary role in active inflammation are increased in 
      gastric mucosa infected with Helicobacter pylori. Cigarette smoking increases the 
      risk of peptic ulcer disease and gastric cancer, whereas alcohol might exert an 
      antibacterial role. The aim of this study was to examine the association between 
      smoking or alcohol consumption and mucosal chemokine mRNA expression in H pylori 
      associated gastritis. METHODS: Gastric biopsy specimens were obtained from 46 
      patients with dyspepsia who were infected with H pylori, and total RNA was 
      extracted. Semiquantitative reverse transcription-polymerase chain reaction 
      (RT-PCR) was performed to quantify the mRNA expression of three C-X-C chemokines 
      (interleukin 8 (IL-8), growth related oncogene alpha (GRO alpha), epithelial 
      neutrophil activating protein 78 (ENA-78)) and two C-C chemokines (regulated on 
      activation normal T cell expressed and secreted (RANTES) and monocyte chemotactic 
      protein 1 (MCP-1)). RESULTS: GRO alpha and ENA-78 mRNA expression was 
      significantly increased (p < 0.05) in 22 smokers compared with 24 non-smokers; 
      however, no difference was seen in the expression of IL-8, RANTES, and MCP-1 
      mRNA. No differences were observed in chemokine mRNA expression in relation to 
      alcohol consumption. CONCLUSIONS: The increased C-X-C chemokine mRNA expression 
      seen in smokers might play a role in inducing enhanced inflammatory activity in 
      gastritis and the consequent severe diseases associated with H pylori infection.
FAU - Shimoyama, T
AU  - Shimoyama T
AD  - First Department of Internal Medicine, Hirosaki University School of Medicine, 5 
      Zaifu-cho, Hirosaki 036-8562, Japan. tsimo-hki@umin.ac.jp
FAU - Everett, S M
AU  - Everett SM
FAU - Fukuda, S
AU  - Fukuda S
FAU - Axon, A T
AU  - Axon AT
FAU - Dixon, M F
AU  - Dixon MF
FAU - Crabtree, J E
AU  - Crabtree JE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Clin Pathol
JT  - Journal of clinical pathology
JID - 0376601
RN  - 0 (CXCL1 protein, human)
RN  - 0 (CXCL5 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Chemokine CXCL1)
RN  - 0 (Chemokine CXCL5)
RN  - 0 (Chemokines)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Growth Substances)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Interleukin-8)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Alcohol Drinking
MH  - Case-Control Studies
MH  - Chemokine CCL2/genetics
MH  - Chemokine CCL5/genetics
MH  - Chemokine CXCL1
MH  - Chemokine CXCL5
MH  - Chemokines/genetics/*metabolism
MH  - *Chemokines, CXC
MH  - Chemotactic Factors/genetics
MH  - Chi-Square Distribution
MH  - Dyspepsia/immunology/*metabolism/microbiology
MH  - Gastric Mucosa/immunology/*metabolism
MH  - Growth Substances/genetics
MH  - Helicobacter Infections/immunology/*metabolism
MH  - *Helicobacter pylori
MH  - Humans
MH  - *Intercellular Signaling Peptides and Proteins
MH  - Interleukin-8/*analogs & derivatives/genetics
MH  - RNA, Messenger/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Smoking/*adverse effects/metabolism
PMC - PMC1731392
EDAT- 2001/04/18 10:00
MHDA- 2001/05/01 10:01
PMCR- 2004/04/01
CRDT- 2001/04/18 10:00
PHST- 2001/04/18 10:00 [pubmed]
PHST- 2001/05/01 10:01 [medline]
PHST- 2001/04/18 10:00 [entrez]
PHST- 2004/04/01 00:00 [pmc-release]
AID - 10.1136/jcp.54.4.332 [doi]
PST - ppublish
SO  - J Clin Pathol. 2001 Apr;54(4):332-4. doi: 10.1136/jcp.54.4.332.