PMID- 11306738
OWN - NLM
STAT- MEDLINE
DCOM- 20010719
LR  - 20110316
IS  - 1011-8934 (Print)
IS  - 1598-6357 (Electronic)
IS  - 1011-8934 (Linking)
VI  - 16
IP  - 2
DP  - 2001 Apr
TI  - Detection of fetal erythroid cells from maternal blood using fluorescence in situ 
      hybridization and liquid culture.
PG  - 145-9
AB  - Fetal nucleated erythrocytes circulating in maternal blood are a potential source 
      of fetal DNA for noninvasive prenatal genetic diagnosis. However, the estimated 
      ratio of fetal to maternal cells is extremely small. In order to enrich these 
      cells, we performed direct culture using a two-phase liquid system. Mononuclear 
      cells were obtained from maternal blood samples at 8-10(+3) weeks of gestation 
      and cultured in the first phase. After 4-5 days, the nonadherent cells were 
      harvested and recultured with erythropoietin in the second phase for another 3-5 
      days. We examined cellular morphology, and counted the number of benzidine- 
      positive cells and the percentage of glycophorin A/CD71 positive erythroid cells. 
      We also did Kleihauer-Betke stain for Hb F, polymerase chain reaction (PCR) for 
      SRY/DYZ1, chromosome analysis, and fluorescence in situ hybridization (FISH). The 
      number of total erythroid cells reached about 0.1 x 10(6)-1.0 x 10(6)/mL with a 
      purity of 84.0-97.3%. Hb F stain showed total erythroid cells of approximately 
      0.4 x 10(4)-9.8 x 10(4)/mL. Male DNA was detected in one case by PCR. In this 
      case, the XY karyotype was confirmed by FISH and amniocentesis. This approach 
      provides enriched source of fetal cells for further prenatal genetic analysis 
      without complicated separation or sorting procedures.
FAU - Han, J Y
AU  - Han JY
AD  - Department of Clinical Pathology, Dong-A University College of Medicine, Pusan, 
      Korea. jyhan@daunet.donga.ac.kr
FAU - Kim, K H
AU  - Kim KH
FAU - Park, J I
AU  - Park JI
FAU - Kim, I H
AU  - Kim IH
FAU - Je, G H
AU  - Je GH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Korea (South)
TA  - J Korean Med Sci
JT  - Journal of Korean medical science
JID - 8703518
SB  - IM
MH  - Cell Culture Techniques/methods
MH  - Cell Separation/*methods
MH  - Chromosome Aberrations/diagnosis
MH  - Chromosome Disorders
MH  - *Erythroblasts
MH  - Female
MH  - Genetic Testing
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - *Maternal-Fetal Exchange
MH  - Pregnancy
MH  - Prenatal Diagnosis/*methods
PMC - PMC3054725
EDAT- 2001/04/18 10:00
MHDA- 2001/07/20 10:01
PMCR- 2001/04/01
CRDT- 2001/04/18 10:00
PHST- 2001/04/18 10:00 [pubmed]
PHST- 2001/07/20 10:01 [medline]
PHST- 2001/04/18 10:00 [entrez]
PHST- 2001/04/01 00:00 [pmc-release]
AID - 200104145 [pii]
AID - 10.3346/jkms.2001.16.2.145 [doi]
PST - ppublish
SO  - J Korean Med Sci. 2001 Apr;16(2):145-9. doi: 10.3346/jkms.2001.16.2.145.