PMID- 11309700
OWN - NLM
STAT- MEDLINE
DCOM- 20010726
LR  - 20191104
IS  - 0037-1963 (Print)
IS  - 0037-1963 (Linking)
VI  - 38
IP  - 2
DP  - 2001 Apr
TI  - Flow cytometry in human leukocyte antigen testing.
PG  - 194-200
AB  - Stem cell transplantation is a recognized treatment for many hematologic 
      malignancies. Human leukocyte antigen (HLA) testing is a key assessment in the 
      evaluation of potential recipients and selection of the appropriate stem cell 
      donor. Over the past several years, technological advances have moved HLA testing 
      from a serologic art form to a sophisticated and high-resolution molecular-based 
      procedure. HLA typing is now performed by DNA-based methods, while flow cytometry 
      has significantly improved our ability to detect HLA antibodies. Although 
      DNA-based typing methods have advanced our understanding of HLA polymorphisms, 
      HLA antibodies still play a role in both stem cell and solid organ 
      transplantation and can lead to failed engraftment or allograft graft rejection, 
      respectively. Flow cytometric crossmatching and HLA antigen-specific 
      microparticle assays represent the new HLA serology. Together, these new 
      technologies are impacting patient outcomes and are expanding our understanding 
      of the immune response to HLA alloantigens. Semin Hematol 38:194-200.
CI  - Copyright 2001 by W.B. Saunders Company.
FAU - Bray, R A
AU  - Bray RA
AD  - Department of Pathology, Emory University Hospital, Atlanta, GA 30322, USA.
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Semin Hematol
JT  - Seminars in hematology
JID - 0404514
RN  - 0 (Histocompatibility Antigens)
SB  - IM
MH  - Animals
MH  - Flow Cytometry/*methods
MH  - Histocompatibility Antigens/analysis
MH  - Histocompatibility Testing/*methods
MH  - Humans
RF  - 17
EDAT- 2001/04/20 10:00
MHDA- 2001/07/28 10:01
CRDT- 2001/04/20 10:00
PHST- 2001/04/20 10:00 [pubmed]
PHST- 2001/07/28 10:01 [medline]
PHST- 2001/04/20 10:00 [entrez]
AID - S0037196301000415 [pii]
AID - 10.1016/s0037-1963(01)90052-6 [doi]
PST - ppublish
SO  - Semin Hematol. 2001 Apr;38(2):194-200. doi: 10.1016/s0037-1963(01)90052-6.