PMID- 11310745
OWN - NLM
STAT- MEDLINE
DCOM- 20011025
LR  - 20121115
IS  - 0894-0282 (Print)
IS  - 0894-0282 (Linking)
VI  - 14
IP  - 4
DP  - 2001 Apr
TI  - A purine-related metabolite negatively regulates fixNOQP expression in 
      Sinorhizobium meliloti by modulation of fixK expression.
PG  - 572-6
AB  - 5-aminoimidazole-4-carboxamide nucleotide (AICAR) is a negative effector of 
      cytochrome terminal oxidase cbb3 production in Rhizobium etli. In this work, the 
      effect of AICAriboside (AICAr), the precursor of AICAR on the expression of the 
      Sinorhizobium meliloti fixNOQP operon encoding the symbiotic terminal oxidase 
      cbb3, was analyzed. AICAr reduced the microaerobic induction levels of fixN-lacZ 
      and fixT-lacZ gene fusions 18- and seven-fold respectively, and both genes were 
      activated by the transcriptional activator FixK. A fixK-lacZ fusion presented 
      14-fold-reduced induction levels in microaerobic cell cultures in the presence of 
      AICAr. AICAr also reduced three-fold the microaerobic expression levels of the 
      nifA-lacZ fusion, whose expression as well as that of fixK is controlled by the 
      two-component system FixL-FixJ. In contrast, AICAr had no effect on the 
      expression levels of a hemA-lacZ fusion. These data suggest that AICAr prevents 
      fixNOQP induction by the inhibition of fixK transcription.
FAU - Soberon, M
AU  - Soberon M
AD  - Instituto de Biotecnologia, Departamento de Biologia Molecular de Plantas, UNAM, 
      Cuernavaca, Mexico. mario@ibt.unam.mx
FAU - Morera, C
AU  - Morera C
FAU - Kondorosi, A
AU  - Kondorosi A
FAU - Lopez, O
AU  - Lopez O
FAU - Miranda, J
AU  - Miranda J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Plant Microbe Interact
JT  - Molecular plant-microbe interactions : MPMI
JID - 9107902
RN  - 0 (Bacterial Proteins)
RN  - 0 (Purines)
RN  - 0 (Ribonucleotides)
RN  - 360-97-4 (Aminoimidazole Carboxamide)
RN  - F0X88YW0YK (AICA ribonucleotide)
SB  - IM
MH  - Aminoimidazole Carboxamide/*analogs & derivatives
MH  - Bacterial Proteins/*genetics
MH  - Gene Expression Regulation, Bacterial/physiology
MH  - *Genes, Bacterial
MH  - Purines/*metabolism
MH  - Ribonucleotides/physiology
MH  - Sinorhizobium meliloti/*genetics
EDAT- 2001/04/20 10:00
MHDA- 2001/10/26 10:01
CRDT- 2001/04/20 10:00
PHST- 2001/04/20 10:00 [pubmed]
PHST- 2001/10/26 10:01 [medline]
PHST- 2001/04/20 10:00 [entrez]
AID - 10.1094/MPMI.2001.14.4.572 [doi]
PST - ppublish
SO  - Mol Plant Microbe Interact. 2001 Apr;14(4):572-6. doi: 
      10.1094/MPMI.2001.14.4.572.