PMID- 11311885
OWN - NLM
STAT- MEDLINE
DCOM- 20020402
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 899
IP  - 1-2
DP  - 2001 Apr 27
TI  - Release of immunoreactive brain-derived neurotrophic factor in the spinal cord of 
      the rat following sciatic nerve transection.
PG  - 240-7
AB  - Using the antibody microprobe method, the sites of spinal release of 
      immunoreactive brain-derived neurotrophic factor (BDNF) was studied in normal 
      rats, and rats with prior sciatic nerve transection. In normal rats, a 
      significant basal release of immunoreactive BDNF was found in the superficial 
      dorsal horn. Following sciatic nerve transection (performed 14 days previously), 
      release of BDNF was found throughout the whole of the dorsal horn, extending into 
      deeper laminae. Electrical stimulation of the ipsilateral sciatic nerve at a 
      strength adequate to excite either A fibres (20 Hz at 2x threshold voltage) or A 
      and C fibres (2 Hz at 20x threshold voltage) did not alter the basal release of 
      immunoreactive BDNF in normal or in nerve-injured rats. The results suggest that 
      BDNF is released from the central terminals of primary afferent fibres, but such 
      release is not solely dependent upon action potential invasion of these 
      terminals. The increased extent of release following nerve transection is 
      consistent with the hypothesis that BDNF plays a role in the central response to 
      peripheral nerve injury.
FAU - Walker, S M
AU  - Walker SM
AD  - Department of Anaesthesia and Pain Management, Royal North Shore Hospital, 2065, 
      New South Wales, St Leonard's, Australia.
FAU - Mitchell, V A
AU  - Mitchell VA
FAU - White, D M
AU  - White DM
FAU - Rush, R A
AU  - Rush RA
FAU - Duggan, A W
AU  - Duggan AW
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/analysis/biosynthesis/*metabolism
MH  - Electric Stimulation
MH  - Posterior Horn Cells/chemistry/metabolism
MH  - Rats
MH  - Sciatic Nerve/*physiology
MH  - Spinal Cord/chemistry/*metabolism
EDAT- 2001/04/20 10:00
MHDA- 2002/04/03 10:01
CRDT- 2001/04/20 10:00
PHST- 2001/04/20 10:00 [pubmed]
PHST- 2002/04/03 10:01 [medline]
PHST- 2001/04/20 10:00 [entrez]
AID - S0006-8993(01)02259-4 [pii]
AID - 10.1016/s0006-8993(01)02259-4 [doi]
PST - ppublish
SO  - Brain Res. 2001 Apr 27;899(1-2):240-7. doi: 10.1016/s0006-8993(01)02259-4.