PMID- 11312606
OWN - NLM
STAT- MEDLINE
DCOM- 20010712
LR  - 20121115
IS  - 1044-7431 (Print)
IS  - 1044-7431 (Linking)
VI  - 17
IP  - 4
DP  - 2001 Apr
TI  - Sustained effects of gene-activated matrices after CNS injury.
PG  - 706-16
AB  - We show that when gene-activated matrices (GAM) are placed between the proximal 
      and distal stumps of severed rat optic nerves, DNA is retained within the GAM, 
      promoting sustained transgene expression in the optic nerve, in the GAM itself, 
      and, more importantly, in axotomized retinal ganglion cells (RGC). Plasmids that 
      encode basic fibroblast growth factor (FGF2), brain-derived neurotrophic factor 
      (BDNF), and neurotrophin-3 (NT3) promote sustained survival of RGC for over 3 
      months after the initial injury. These findings suggest that immobilized DNA 
      implanted into a CNS lesion will be delivered by axon terminal uptake and 
      retrograde transport to axotomized neurons. GAM may therefore be a useful agent 
      for promoting sustained neuron survival and axon regeneration. Whether further 
      optimization of the matrices, plasmids, promoters, and genes present in the GAM 
      will promote even more survival or, alternatively, axon regeneration remains to 
      be determined.
CI  - Copyright 2001 Academic Press.
FAU - Berry, M
AU  - Berry M
AD  - Centre for Neuroscience, Neural Damage and Repair, GKT (Guy's Campus), Hodgkin 
      Building, London Bridge, London, SE1 1UL, United Kingdom.
FAU - Gonzalez, A M
AU  - Gonzalez AM
FAU - Clarke, W
AU  - Clarke W
FAU - Greenlees, L
AU  - Greenlees L
FAU - Barrett, L
AU  - Barrett L
FAU - Tsang, W
AU  - Tsang W
FAU - Seymour, L
AU  - Seymour L
FAU - Bonadio, J
AU  - Bonadio J
FAU - Logan, A
AU  - Logan A
FAU - Baird, A
AU  - Baird A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cell Neurosci
JT  - Molecular and cellular neurosciences
JID - 9100095
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neurotrophin 3)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
MH  - Animals
MH  - Axotomy
MH  - Brain-Derived Neurotrophic Factor/genetics
MH  - Cell Line
MH  - Cell Survival/physiology
MH  - Cricetinae
MH  - Female
MH  - Fibroblast Growth Factor 2/*genetics
MH  - Gene Expression Regulation/*physiology
MH  - Genetic Therapy/*methods
MH  - Kidney/cytology
MH  - Neurotrophin 3/genetics
MH  - Optic Nerve/cytology/*physiology
MH  - Optic Nerve Injuries/*therapy
MH  - Plasmids
MH  - Rats
MH  - Rats, Wistar
MH  - Retinal Ganglion Cells/cytology/physiology
MH  - Transfection/methods
EDAT- 2001/04/21 10:00
MHDA- 2001/07/13 10:01
CRDT- 2001/04/21 10:00
PHST- 2001/04/21 10:00 [pubmed]
PHST- 2001/07/13 10:01 [medline]
PHST- 2001/04/21 10:00 [entrez]
AID - S1044-7431(01)90975-3 [pii]
AID - 10.1006/mcne.2001.0975 [doi]
PST - ppublish
SO  - Mol Cell Neurosci. 2001 Apr;17(4):706-16. doi: 10.1006/mcne.2001.0975.