PMID- 11313501
OWN - NLM
STAT- MEDLINE
DCOM- 20010531
LR  - 20240322
IS  - 0036-8075 (Print)
IS  - 1095-9203 (Electronic)
IS  - 0036-8075 (Linking)
VI  - 292
IP  - 5520
DP  - 2001 May 18
TI  - A GDP/GTP exchange factor involved in linking a spatial landmark to cell 
      polarity.
PG  - 1376-8
AB  - In both animal and yeast cells, signaling pathways involving small guanosine 
      triphosphatases (GTPases) regulate polarized organization of the actin 
      cytoskeleton. In the budding yeast Saccharomyces cerevisiae, the Ras-like GTPase 
      Bud1/Rsr1 and its guanosine 5'-diphosphate (GDP)/guanosine 5'-triphosphate (GTP) 
      exchange factor Bud5 are involved in the selection of a specific site for growth, 
      thus determining cell polarity. We found that Bud5 is localized at the cell 
      division site and the presumptive bud site. Its localization is dependent on 
      potential cellular landmarks, such as Bud3 and Axl2/Bud10 in haploid cells and 
      Bud8 and Bud9 in diploid cells. Bud5 also physically interacts with Axl2/Bud10, a 
      transmembrane glycoprotein, suggesting that a receptor-like transmembrane protein 
      recruits a GDP/GTP exchange factor to connect an intrinsic spatial signal to 
      oriented cell growth.
FAU - Kang, P J
AU  - Kang PJ
AD  - Department of Molecular Genetics, The Ohio State University, Columbus, OH 
      43210-1292, USA.
FAU - Sanson, A
AU  - Sanson A
FAU - Lee, B
AU  - Lee B
FAU - Park, H O
AU  - Park HO
LA  - eng
GR  - R01 GM056997/GM/NIGMS NIH HHS/United States
GR  - R01 GM056997-02/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20010419
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Axl2 protein, S cerevisiae)
RN  - 0 (BUD3 protein, S cerevisiae)
RN  - 0 (BUD5 protein, S cerevisiae)
RN  - 0 (Fungal Proteins)
RN  - 0 (Guanine Nucleotide Exchange Factors)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 146-91-8 (Guanosine Diphosphate)
RN  - 86-01-1 (Guanosine Triphosphate)
SB  - IM
MH  - Cell Division
MH  - *Cell Polarity
MH  - Diploidy
MH  - Fungal Proteins/genetics/*metabolism
MH  - Genes, Fungal/genetics
MH  - Genotype
MH  - Guanine Nucleotide Exchange Factors
MH  - Guanosine Diphosphate/metabolism
MH  - Guanosine Triphosphate/metabolism
MH  - Haploidy
MH  - Membrane Glycoproteins/genetics/metabolism
MH  - Mutation/genetics
MH  - Precipitin Tests
MH  - Protein Binding
MH  - Recombinant Fusion Proteins/metabolism
MH  - Saccharomyces cerevisiae/*cytology/genetics/*metabolism
MH  - *Saccharomyces cerevisiae Proteins
PMC - PMC4386611
MID - NIHMS285186
EDAT- 2001/04/21 10:00
MHDA- 2001/06/02 10:01
PMCR- 2015/04/06
CRDT- 2001/04/21 10:00
PHST- 2001/04/21 10:00 [pubmed]
PHST- 2001/06/02 10:01 [medline]
PHST- 2001/04/21 10:00 [entrez]
PHST- 2015/04/06 00:00 [pmc-release]
AID - 1060360 [pii]
AID - 10.1126/science.1060360 [doi]
PST - ppublish
SO  - Science. 2001 May 18;292(5520):1376-8. doi: 10.1126/science.1060360. Epub 2001 
      Apr 19.